Activation of endogenous protein phosphatase 1 enhances the calcium sensitivity of the ryanodine receptor type 2 in murine ventricular cardiomyocytes.

KEY POINTS
Increased protein phosphatase 1 (PP-1) activity was found in the late stages of human heart failure. Although PP-1 has been studied extensively, a detailed understanding of its role in excitation-contraction coupling mechanism, in normal and diseased heart, remains elusive. This work examines the functional effects of the activities of PP-1 on the release of Ca2 + local events in ventricular cardiomyocytes, using the activation peptide (PDP3) for the stimulation of endogenous PP-1 protein. We reported that the acute de-phosphorylation may increase the sensitivity of RyR2 to Ca2 + channels in situ, and that RyR2-serine2808 phosphorylation sites can mediate the process. Our approach unmask the functional importance of PP-1 in the regulation of RyR2 activity, suggesting a potential role in the generation of the pathophysiology of the sarcoplasmic reticulum Ca2 + leak at the heart sick.


ABSTRACT
Changes heart Ryanodine receptor (RyR2) phosphorylation was considered a disease of regulation and post-translational modification of proteins related matters. The extent of phosphorylation of RyR2 is mainly determined by the balance of the activity of protein kinases and phosphatases, respectively. Increased protein phosphatase-1 (PP-1) activity has been observed in heart failure, but the role of this enzyme in the regulation of intracellular Ca2 + handling the remains poorly understood.

To determine the physiological and significance of the pathophysiology of increased activity of PP-1, we investigate how the PP-1 catalytic subunit (PP-1c) alter Ca2 + sparks in cardiomyocytes permeabilized and we also apply PP-1-disturbing peptide (PDP3) to specifically activate endogenous PP-1, including one anchored in the RyR2 macromolecular complex.

We compared the wild type (WT) and transgenic mice in which the normally highly phosphorylated RyR2 site-S2808 has been ablated to investigate his involvement in RyR2 modulation (S2808A + / +). In WT myocytes, PP-1 increased the frequency of Ca2 + spark (CaSpF) by 2-fold, followed by depletion of sarcoplasmic reticulum (SR) Ca2 + stores. Similarly, PDP3 temporarily increase and decrease the frequency spark SR Ca2 + load. RyR2 Ca2 + sensitivity, as assessed by analysis of Ca2 + spark recovery, increase with PDP3 when compared to the negative control peptide. S2808A + / + cardiomyocytes do not respond well PP-1c and treatment PDP3.

Our results showed an increase in Ca 2+ sensitivity of RyR2 in the de-phosphorylation by PP-1. Furthermore, we confirmed the S2808 as a target site for PP-1 and as a potential relationship between the modulation RyR2s and cellular responses. This article is protected by copyright. All rights reserved.

Activation of endogenous <em>protein</em> <em>phosphatase</em> <em>1</em> enhances the calcium sensitivity of the ryanodine receptor type 2 in murine ventricular cardiomyocytes.
Activation of endogenous protein phosphatase 1 enhances the calcium sensitivity of the ryanodine receptor type 2 in murine ventricular cardiomyocytes.

Phactr one set Slack (KCNT one ) channels through protein phosphatase one (PP one ).

The Slack (KCNT1) gene encodes sodium-activated potassium channels are abundant expressed in the central nervous system. human mutations alter channel function Slack, resulting in epilepsy and intellectual disability. The majority of disease-causing mutations located in the cytoplasmic C-terminus extended channels and the result is increased current Slack Slack. Previous experiments have shown that the C-terminus of the protein channel Slack bind a number of cytoplasmic signaling.

PPP3R1 Antibody

46646-100ul 100ul
EUR 302.4

PPP3R1 Antibody

45818-100ul 100ul
EUR 302.4

PPP3R1 Antibody

45818-50ul 50ul
EUR 224.4

PPP3R1 antibody

70R-19489 50 ul
EUR 522
Description: Rabbit polyclonal PPP3R1 antibody

PPP3R1 antibody

70R-4295 50 ug
EUR 560.4
Description: Rabbit polyclonal PPP3R1 antibody

PPP3R1 Antibody

ABD9292 100 ug
EUR 525.6

PPP3R1 Antibody

DF9292 200ul
EUR 420

PPP3R1 Antibody

1-CSB-PA018575GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against PPP3R1. Recognizes PPP3R1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

PPP3R1 Antibody

1-CSB-PA018575LA01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP3R1. Recognizes PPP3R1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000

PPP3R1 Rabbit pAb

A0954-100ul 100 ul
EUR 369.6

PPP3R1 Rabbit pAb

A0954-200ul 200 ul
EUR 550.8

PPP3R1 Rabbit pAb

A0954-20ul 20 ul
EUR 219.6

PPP3R1 Rabbit pAb

A0954-50ul 50 ul
EUR 267.6

anti- PPP3R1 antibody

FNab06731 100µg
EUR 606.3
Description: Antibody raised against PPP3R1

PPP3R1 cloning plasmid

CSB-CL018575HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the PPP3R1 gene.

PPP3R1 Blocking Peptide

33R-6049 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of PPP3R1 antibody, catalog no. 70R-4295

PPP3R1 Blocking Peptide

DF9292-BP 1mg
EUR 234

PPP3R1 Polyclonal Antibody

A60394
  • EUR 684.66
  • Ask for price
  • Ask for price
  • EUR 423.50
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

PPP3R1 Polyclonal Antibody

A72156
  • EUR 302.50
  • EUR 423.50
  • 50 ul
  • 100 ul

PPP3R1 Protein (Recombinant)

20-abx073192
  • EUR 4101.60
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

PPP3R1 Conjugated Antibody

C45818 100ul
EUR 476.4

PPP3R1 Conjugated Antibody

C46646 100ul
EUR 476.4

PPP3R1 protein (His tag)

80R-1659 100 ug
EUR 366
Description: Purified recombinant Human PPP3R1 protein

PPP3R1 ELISA KIT|Human

EF005281 96 Tests
EUR 826.8

Human PPP3R1 ELISA Kit

ELA-E1323h 96 Tests
EUR 988.8

Rat PPP3R1 shRNA Plasmid

20-abx985707
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse PPP3R1 shRNA Plasmid

20-abx972178
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Human PPP3R1 shRNA Plasmid

20-abx953713
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

PPP3R1 Antibody, HRP conjugated

1-CSB-PA018575LB01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP3R1. Recognizes PPP3R1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

PPP3R1 Recombinant Protein (Rat)

RP221843 100 ug Ask for price

Calcineurin B Antibody / PPP3R1

V8029-100UG 100 ug
EUR 349.3
Description: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Calcineurin B Antibody / PPP3R1

V8029-20UG 20 ug
EUR 153.3
Description: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Calcineurin B Antibody / PPP3R1

V8029SAF-100UG 100 ug
EUR 349.3
Description: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Calcineurin B Antibody / PPP3R1

V8030-100UG 100 ug
EUR 349.3
Description: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Calcineurin B Antibody / PPP3R1

V8030-20UG 20 ug
EUR 153.3
Description: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Calcineurin B Antibody / PPP3R1

V8030SAF-100UG 100 ug
EUR 349.3
Description: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

PPP3R1 Antibody, FITC conjugated

1-CSB-PA018575LC01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP3R1. Recognizes PPP3R1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

PPP3R1 Recombinant Protein (Human)

RP024436 100 ug Ask for price

PPP3R1 Recombinant Protein (Mouse)

RP164066 100 ug Ask for price

PPP3R1 Antibody, Biotin conjugated

1-CSB-PA018575LD01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP3R1. Recognizes PPP3R1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Ppp3r1 ORF Vector (Rat) (pORF)

ORF073949 1.0 ug DNA
EUR 607.2

PPP3R1 ORF Vector (Human) (pORF)

ORF008146 1.0 ug DNA
EUR 114

Ppp3r1 ORF Vector (Mouse) (pORF)

ORF054690 1.0 ug DNA
EUR 607.2

[One Step] PPP3R1 Antibody Kit

RK05683 50 ul
EUR 288

PPP3R1 ELISA Kit (Rat) (OKEH00948)

OKEH00948 96 Wells
EUR 794.4
Description: Description of target: regulatory subunit of the enzyme that catalyzes the calcium-dependent removal of serine- and threonine-bound phosphate groups [RGD, Feb 2006];Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.086 ng/mL

PPP3R1 ELISA Kit (Mouse) (OKCD02818)

OKCD02818 96 Wells
EUR 1028.4
Description: Description of target: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. ;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.047 ng/mL

PPP3R1 ELISA Kit (Mouse) (OKEH07121)

OKEH07121 96 Wells
EUR 794.4
Description: Description of target: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. ;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.085 ng/mL

PPP3R1 ELISA Kit (Human) (OKEH00947)

OKEH00947 96 Wells
EUR 794.4
Description: Description of target: ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.067 ng/mL

PPP3R1 Polyclonal Antibody, HRP Conjugated

A60397
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

PPP3R1 Polyclonal Antibody, FITC Conjugated

A60396
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

PPP3R1 Polyclonal Antibody, Biotin Conjugated

A60395
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

Ppp3r1 sgRNA CRISPR Lentivector set (Rat)

K6874001 3 x 1.0 ug
EUR 406.8

PPP3R1 sgRNA CRISPR Lentivector set (Human)

K1709301 3 x 1.0 ug
EUR 406.8

PPP3R1 3'UTR GFP Stable Cell Line

TU068697 1.0 ml
EUR 1825.2

Ppp3r1 3'UTR GFP Stable Cell Line

TU266693 1.0 ml Ask for price

PPP3R1 Protein Vector (Rat) (pPM-C-HA)

PV295796 500 ng
EUR 723.6

PPP3R1 Protein Vector (Rat) (pPB-C-His)

PV295794 500 ng
EUR 723.6

PPP3R1 Protein Vector (Rat) (pPB-N-His)

PV295795 500 ng
EUR 723.6

PPP3R1 Protein Vector (Rat) (pPM-C-His)

PV295797 500 ng
EUR 723.6

PPP3R1 Protein Vector (Human) (pPM-C-HA)

PV032583 500 ng
EUR 394.8

PPP3R1 Protein Vector (Mouse) (pPM-C-HA)

PV218760 500 ng
EUR 723.6

PPP3R1 Protein Vector (Human) (pPB-C-His)

PV032581 500 ng
EUR 394.8

PPP3R1 Protein Vector (Human) (pPB-N-His)

PV032582 500 ng
EUR 394.8

PPP3R1 Protein Vector (Human) (pPM-C-His)

PV032584 500 ng
EUR 394.8

PPP3R1 Protein Vector (Mouse) (pPB-C-His)

PV218758 500 ng
EUR 723.6

PPP3R1 Protein Vector (Mouse) (pPB-N-His)

PV218759 500 ng
EUR 723.6

PPP3R1 Protein Vector (Mouse) (pPM-C-His)

PV218761 500 ng
EUR 723.6

PPP3R1 3'UTR Luciferase Stable Cell Line

TU018697 1.0 ml
EUR 1825.2

Ppp3r1 3'UTR Luciferase Stable Cell Line

TU216693 1.0 ml Ask for price

Ppp3r1 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6874002 1.0 ug DNA
EUR 184.8

Ppp3r1 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6874003 1.0 ug DNA
EUR 184.8

Ppp3r1 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6874004 1.0 ug DNA
EUR 184.8

Calcineurin Subunit B Type 1 (PPP3R1) Antibody

20-abx217914
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Calcineurin Subunit B Type 1 (PPP3R1) Antibody

20-abx114854
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Calcineurin Subunit B Type 1 (PPP3R1) Antibody

20-abx000913
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Calcineurin Subunit B Type 1 (PPP3R1) Antibody

abx236731-100ug 100 ug
EUR 577.2

Calcineurin Subunit B Type 1 (PPP3R1) Antibody

abx122626-100ug 100 ug
EUR 469.2

Calcineurin Subunit B Type 1 (PPP3R1) Antibody

20-abx302644
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

PPP3R1 sgRNA CRISPR Lentivector (Human) (Target 1)

K1709302 1.0 ug DNA
EUR 184.8

PPP3R1 sgRNA CRISPR Lentivector (Human) (Target 2)

K1709303 1.0 ug DNA
EUR 184.8

PPP3R1 sgRNA CRISPR Lentivector (Human) (Target 3)

K1709304 1.0 ug DNA
EUR 184.8

Recombinant Human PPP3R1 Protein, His, E.coli-1mg

QP13129-1mg 1mg
EUR 3308.4

Recombinant Human PPP3R1 Protein, His, E.coli-5ug

QP13129-5ug 5ug
EUR 186

Recombinant Human PPP3R1 Protein, His, E.coli-20ug

QP13129-20ug 20ug
EUR 241.2

Monoclonal PPP3R1 Antibody (monoclonal) (M01), Clone: 40

AMM07304G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human PPP3R1 (monoclonal) (M01). The antibodies are raised in mouse and are from clone 40. This antibody is applicable in WB and IF, E

Polyclonal PPP3R1 / Calcineurin B Antibody (aa7-37)

AMM07318G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PPP3R1 / Calcineurin B (aa7-37). This antibody is tested and proven to work in the following applications:

Calcineurin Subunit B Type 1 (PPP3R1) Antibody (HRP)

20-abx304942
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Calcineurin Subunit B Type 1 (PPP3R1) Antibody (FITC)

20-abx304943
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

PPP3R1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV676135 1.0 ug DNA
EUR 616.8

PPP3R1 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV676139 1.0 ug DNA
EUR 616.8

Calcineurin Subunit B Type 1 (PPP3R1) Antibody (Biotin)

20-abx304944
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

PPP3R1 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV676140 1.0 ug DNA
EUR 616.8

Rat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E02C2398-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E02C2398-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E02C2398-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Cow Calcineurin Subunit B Type 1 (PPP3R1) ELISA Kit

abx516389-96tests 96 tests
EUR 1093.2

Dog Calcineurin subunit B type 1(PPP3R1) ELISA kit

E08C2398-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Calcineurin subunit B type 1(PPP3R1) ELISA kit

E08C2398-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Calcineurin subunit B type 1(PPP3R1) ELISA kit

E08C2398-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Ppp3r1/ Calcineurin subunit B type 1 ELISA Kit

E0789Ra 1 Kit
EUR 685.2

Rat Ppp3r1(Calcineurin subunit B type 1) ELISA Kit

ER0503 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Rat Calcineurin Subunit B Type 1 (PPP3R1) ELISA Kit

abx256480-96tests 96 tests
EUR 801.6

Pig Calcineurin subunit B type 1(PPP3R1) ELISA kit

E07C2398-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Calcineurin subunit B type 1(PPP3R1) ELISA kit

E07C2398-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Calcineurin subunit B type 1(PPP3R1) ELISA kit

E07C2398-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E06C2398-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E06C2398-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E06C2398-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human PPP3R1/ Calcineurin subunit B type 1 ELISA Kit

E2016Hu 1 Kit
EUR 685.2

Mouse Calcineurin subunit B type 1, Ppp3r1 ELISA KIT

ELI-04365m 96 Tests
EUR 1038

Human Calcineurin subunit B type 1, PPP3R1 ELISA KIT

ELI-04367h 96 Tests
EUR 988.8

Human Calcineurin Subunit B Type 1 (PPP3R1) ELISA Kit

abx250774-96tests 96 tests
EUR 801.6

Mouse Calcineurin Subunit B Type 1 (PPP3R1) ELISA Kit

abx570694-96tests 96 tests
EUR 801.6

Human Calcineurin subunit B type 1(PPP3R1) ELISA kit

E01C2398-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Calcineurin subunit B type 1(PPP3R1) ELISA kit

E01C2398-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Calcineurin subunit B type 1(PPP3R1) ELISA kit

E01C2398-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Calcineurin subunit B type 1(PPP3R1) ELISA kit

E03C2398-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Calcineurin subunit B type 1(PPP3R1) ELISA kit

E03C2398-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Calcineurin subunit B type 1(PPP3R1) ELISA kit

E03C2398-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human PPP3R1(Calcineurin subunit B type 1) ELISA Kit

EH1491 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Antibody

20-abx178172
  • EUR 1479.60
  • EUR 710.40
  • 1 mg
  • 200 ug

Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Antibody

20-abx178173
  • EUR 1479.60
  • EUR 710.40
  • 1 mg
  • 200 ug

Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Antibody

20-abx129114
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Bovine Calcineurin subunit B type 1, PPP3R1 ELISA KIT

ELI-04364b 96 Tests
EUR 1113.6

Rabbit Calcineurin subunit B type 1(PPP3R1) ELISA kit

E04C2398-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Calcineurin subunit B type 1(PPP3R1) ELISA kit

E04C2398-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Calcineurin subunit B type 1(PPP3R1) ELISA kit

E04C2398-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Calcineurin subunit B type 1(PPP3R1) ELISA kit

E09C2398-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Calcineurin subunit B type 1(PPP3R1) ELISA kit

E09C2398-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Calcineurin subunit B type 1(PPP3R1) ELISA kit

E09C2398-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Recombinant Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1)

4-RPE020Hu01
  • EUR 560.83
  • EUR 273.60
  • EUR 1773.12
  • EUR 671.04
  • EUR 1222.08
  • EUR 451.20
  • EUR 4252.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Protein Phosphatase 3, Regulatory Subunit 1 expressed in: E.coli

ELISA kit for Rat Calcineurin subunit B type 1 (PPP3R1)

KTE100456-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Rat Calcineurin subunit B type 1 (PPP3R1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Calcineurin subunit B type 1 (PPP3R1)

KTE100456-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Rat Calcineurin subunit B type 1 (PPP3R1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Calcineurin subunit B type 1 (PPP3R1)

KTE100456-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Rat Calcineurin subunit B type 1 (PPP3R1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Guinea pig Calcineurin subunit B type 1(PPP3R1) ELISA kit

E05C2398-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Calcineurin subunit B type 1(PPP3R1) ELISA kit

E05C2398-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Calcineurin subunit B type 1(PPP3R1) ELISA kit

E05C2398-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Ppp3r1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6874005 3 x 1.0 ug
EUR 451.2

Human Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Protein

20-abx166252
  • EUR 777.60
  • EUR 326.40
  • EUR 2397.60
  • EUR 927.60
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Protein

20-abx654868
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Protein

20-abx654869
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

PPP3R1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K1709305 3 x 1.0 ug
EUR 451.2

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

DLR-PPP3R1-Ra-48T 48T
EUR 658.8
Description: A sandwich quantitative ELISA assay kit for detection of Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) in samples from tissue homogenates or other biological fluids.

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

DLR-PPP3R1-Ra-96T 96T
EUR 861.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) in samples from tissue homogenates or other biological fluids.

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RD-PPP3R1-Ra-48Tests 48 Tests
EUR 668.4

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RD-PPP3R1-Ra-96Tests 96 Tests
EUR 930

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RDR-PPP3R1-Ra-48Tests 48 Tests
EUR 699.6

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RDR-PPP3R1-Ra-96Tests 96 Tests
EUR 973.2

Ppp3r1 ELISA Kit| Rat Calcineurin subunit B type 1 ELISA Kit

EF017341 96 Tests
EUR 826.8

Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) CLIA Kit

20-abx494477
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

20-abx154571
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

One is Phactr1, actin-binding protein that recruits protein phosphatase 1 (PP1) to specific phosphoprotein substrates. Using co-immunoprecipitation, we found that Phactr1 required to connect the channel to actin. Using a clamp recordings, we found that co-expression of wild-type channels Phactr1 with Slack reduce current amplitude but had no effect on the channel Slack where preserved PKC phosphorylation sites (S407), which regulates the current amplitude has been mutated.