Activation of endogenous protein phosphatase 1 enhances the calcium sensitivity of the ryanodine receptor type 2 in murine ventricular cardiomyocytes.

KEY POINTS
Increased protein phosphatase 1 (PP-1) activity was found in the late stages of human heart failure. Although PP-1 has been studied extensively, a detailed understanding of its role in excitation-contraction coupling mechanism, in normal and diseased heart, remains elusive. This work examines the functional effects of the activities of PP-1 on the release of Ca2 + local events in ventricular cardiomyocytes, using the activation peptide (PDP3) for the stimulation of endogenous PP-1 protein. We reported that the acute de-phosphorylation may increase the sensitivity of RyR2 to Ca2 + channels in situ, and that RyR2-serine2808 phosphorylation sites can mediate the process. Our approach unmask the functional importance of PP-1 in the regulation of RyR2 activity, suggesting a potential role in the generation of the pathophysiology of the sarcoplasmic reticulum Ca2 + leak at the heart sick.


ABSTRACT
Changes heart Ryanodine receptor (RyR2) phosphorylation was considered a disease of regulation and post-translational modification of proteins related matters. The extent of phosphorylation of RyR2 is mainly determined by the balance of the activity of protein kinases and phosphatases, respectively. Increased protein phosphatase-1 (PP-1) activity has been observed in heart failure, but the role of this enzyme in the regulation of intracellular Ca2 + handling the remains poorly understood.

To determine the physiological and significance of the pathophysiology of increased activity of PP-1, we investigate how the PP-1 catalytic subunit (PP-1c) alter Ca2 + sparks in cardiomyocytes permeabilized and we also apply PP-1-disturbing peptide (PDP3) to specifically activate endogenous PP-1, including one anchored in the RyR2 macromolecular complex.

We compared the wild type (WT) and transgenic mice in which the normally highly phosphorylated RyR2 site-S2808 has been ablated to investigate his involvement in RyR2 modulation (S2808A + / +). In WT myocytes, PP-1 increased the frequency of Ca2 + spark (CaSpF) by 2-fold, followed by depletion of sarcoplasmic reticulum (SR) Ca2 + stores. Similarly, PDP3 temporarily increase and decrease the frequency spark SR Ca2 + load. RyR2 Ca2 + sensitivity, as assessed by analysis of Ca2 + spark recovery, increase with PDP3 when compared to the negative control peptide. S2808A + / + cardiomyocytes do not respond well PP-1c and treatment PDP3.

Our results showed an increase in Ca 2+ sensitivity of RyR2 in the de-phosphorylation by PP-1. Furthermore, we confirmed the S2808 as a target site for PP-1 and as a potential relationship between the modulation RyR2s and cellular responses. This article is protected by copyright. All rights reserved.

Activation of endogenous <em>protein</em> <em>phosphatase</em> <em>1</em> enhances the calcium sensitivity of the ryanodine receptor type 2 in murine ventricular cardiomyocytes.
Activation of endogenous protein phosphatase 1 enhances the calcium sensitivity of the ryanodine receptor type 2 in murine ventricular cardiomyocytes.

Phactr one set Slack (KCNT one ) channels through protein phosphatase one (PP one ).

The Slack (KCNT1) gene encodes sodium-activated potassium channels are abundant expressed in the central nervous system. human mutations alter channel function Slack, resulting in epilepsy and intellectual disability. The majority of disease-causing mutations located in the cytoplasmic C-terminus extended channels and the result is increased current Slack Slack. Previous experiments have shown that the C-terminus of the protein channel Slack bind a number of cytoplasmic signaling.

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RD-PPP3R1-Ra-48Tests 48 Tests
EUR 557

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RD-PPP3R1-Ra-96Tests 96 Tests
EUR 775

Human Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

DLR-PPP3R1-Hu-48T 48T
EUR 517
Description: A sandwich quantitative ELISA assay kit for detection of Human Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) in samples from tissue homogenates or other biological fluids.

Human Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

DLR-PPP3R1-Hu-96T 96T
EUR 673
Description: A sandwich quantitative ELISA assay kit for detection of Human Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) in samples from tissue homogenates or other biological fluids.

Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

DLR-PPP3R1-Mu-48T 48T
EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) in samples from tissue homogenates or other biological fluids.

Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

DLR-PPP3R1-Mu-96T 96T
EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) in samples from tissue homogenates or other biological fluids.

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

DLR-PPP3R1-Ra-48T 48T
EUR 549
Description: A sandwich quantitative ELISA assay kit for detection of Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) in samples from tissue homogenates or other biological fluids.

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

DLR-PPP3R1-Ra-96T 96T
EUR 718
Description: A sandwich quantitative ELISA assay kit for detection of Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) in samples from tissue homogenates or other biological fluids.

Human Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RDR-PPP3R1-Hu-48Tests 48 Tests
EUR 544

Human Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RDR-PPP3R1-Hu-96Tests 96 Tests
EUR 756

Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RDR-PPP3R1-Mu-48Tests 48 Tests
EUR 557

Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RDR-PPP3R1-Mu-96Tests 96 Tests
EUR 774

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RDR-PPP3R1-Ra-48Tests 48 Tests
EUR 583

Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

RDR-PPP3R1-Ra-96Tests 96 Tests
EUR 811

Ppp3r1/ Rat Ppp3r1 ELISA Kit

ELI-04366r 96 Tests
EUR 886

PPP3R1 Antibody

1-CSB-PA018575GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against PPP3R1. Recognizes PPP3R1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

PPP3R1 Antibody

1-CSB-PA018575LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP3R1. Recognizes PPP3R1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000

PPP3R1 siRNA

20-abx929572
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
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PPP3R1 siRNA

20-abx929573
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  • 15 nmol
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PPP3R1 siRNA

20-abx904202
  • EUR 551.00
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  • 15 nmol
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PPP3R1 Antibody

45818-100ul 100ul
EUR 252

PPP3R1 Antibody

45818-50ul 50ul
EUR 187

PPP3R1 Antibody

46646-100ul 100ul
EUR 252

PPP3R1 Antibody

ABD9292 100 ug
EUR 438

PPP3R1 Antibody

DF9292 200ul
EUR 304
Description: PPP3R1 Antibody detects endogenous levels of total PPP3R1.

PPP3R1 antibody

70R-19489 50 ul
EUR 435
Description: Rabbit polyclonal PPP3R1 antibody

PPP3R1 antibody

70R-4295 50 ug
EUR 467
Description: Rabbit polyclonal PPP3R1 antibody

PPP3R1 cloning plasmid

CSB-CL018575HU-10ug 10ug
EUR 233
Description: A cloning plasmid for the PPP3R1 gene.

Anti-PPP3R1 antibody

STJ25094 100 µl
EUR 277

PPP3R1 Protein (Recombinant)

20-abx073192
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

PPP3R1 Polyclonal Antibody

A60394 100 µg
EUR 570.55
Description: The best epigenetics products

PPP3R1 Rabbit pAb

A0954-100ul 100 ul
EUR 308

PPP3R1 Rabbit pAb

A0954-200ul 200 ul
EUR 459

PPP3R1 Rabbit pAb

A0954-20ul 20 ul
EUR 183

PPP3R1 Rabbit pAb

A0954-50ul 50 ul
EUR 223

anti- PPP3R1 antibody

FNab06731 100µg
EUR 505.25
Description: Antibody raised against PPP3R1

PPP3R1 Conjugated Antibody

C45818 100ul
EUR 397

PPP3R1 Conjugated Antibody

C46646 100ul
EUR 397

PPP3R1 Blocking Peptide

DF9292-BP 1mg
EUR 195

PPP3R1 Blocking Peptide

33R-6049 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of PPP3R1 antibody, catalog no. 70R-4295

Anti-PPP3R1 antibody

PAab06731 100 ug
EUR 355

PPP3R1 Antibody, HRP conjugated

1-CSB-PA018575LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP3R1. Recognizes PPP3R1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

PPP3R1 Antibody, FITC conjugated

1-CSB-PA018575LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
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Description: A polyclonal antibody against PPP3R1. Recognizes PPP3R1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

PPP3R1 Antibody, Biotin conjugated

1-CSB-PA018575LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
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Description: A polyclonal antibody against PPP3R1. Recognizes PPP3R1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Mouse PPP3R1 shRNA Plasmid

20-abx972178
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
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Rat PPP3R1 shRNA Plasmid

20-abx985707
  • EUR 801.00
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Human PPP3R1 shRNA Plasmid

20-abx953713
  • EUR 801.00
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Human PPP3R1 ELISA Kit

ELA-E1323h 96 Tests
EUR 824

PPP3R1 ELISA KIT|Human

EF005281 96 Tests
EUR 689

PPP3R1 protein (His tag)

80R-1659 100 ug
EUR 305
Description: Purified recombinant Human PPP3R1 protein

pcDNA3.1-Flag-PPP3R1 Plasmid

PVTB01191-2a 2 ug
EUR 356

pCMV-SPORT6-PPP3R1 Plasmid

PVT16302 2 ug
EUR 325

PPP3R1 Recombinant Protein (Human)

RP024436 100 ug Ask for price

PPP3R1 Recombinant Protein (Mouse)

RP164066 100 ug Ask for price

PPP3R1 Recombinant Protein (Rat)

RP221843 100 ug Ask for price

[One Step] PPP3R1 Antibody Kit

RK05683 50 ul
EUR 240

PPP3R1 Polyclonal Antibody, Biotin Conjugated

A60395 100 µg
EUR 570.55
Description: kits suitable for this type of research

PPP3R1 Polyclonal Antibody, FITC Conjugated

A60396 100 µg
EUR 570.55
Description: fast delivery possible

PPP3R1 Polyclonal Antibody, HRP Conjugated

A60397 100 µg
EUR 570.55
Description: reagents widely cited

PPP3R1 ELISA Kit (Human) (OKEH00947)

OKEH00947 96 Wells
EUR 662
Description: Description of target: ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.067 ng/mL

PPP3R1 ELISA Kit (Rat) (OKEH00948)

OKEH00948 96 Wells
EUR 662
Description: Description of target: regulatory subunit of the enzyme that catalyzes the calcium-dependent removal of serine- and threonine-bound phosphate groups [RGD, Feb 2006];Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.086 ng/mL

PPP3R1 ELISA Kit (Mouse) (OKEH07121)

OKEH07121 96 Wells
EUR 662
Description: Description of target: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. ;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.085 ng/mL

PPP3R1 ELISA Kit (Mouse) (OKCD02818)

OKCD02818 96 Wells
EUR 857
Description: Description of target: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. ;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.047 ng/mL

Ppp3r1 ORF Vector (Rat) (pORF)

ORF073949 1.0 ug DNA
EUR 506

PPP3R1 ORF Vector (Human) (pORF)

ORF008146 1.0 ug DNA
EUR 95

pECMV-Ppp3r1-m-FLAG Plasmid

PVT15705 2 ug
EUR 325

Ppp3r1 ORF Vector (Mouse) (pORF)

ORF054690 1.0 ug DNA
EUR 506

PPP3R1 sgRNA CRISPR Lentivector set (Human)

K1709301 3 x 1.0 ug
EUR 339

Ppp3r1 sgRNA CRISPR Lentivector set (Rat)

K6874001 3 x 1.0 ug
EUR 339

Monoclonal PPP3R1 Antibody (monoclonal) (M01), Clone: 40

AMM07304G 0.1mg
EUR 484
Description: A Monoclonal antibody against Human PPP3R1 (monoclonal) (M01). The antibodies are raised in mouse and are from clone 40. This antibody is applicable in WB and IF, E

Polyclonal PPP3R1 / Calcineurin B Antibody (aa7-37)

AMM07318G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PPP3R1 / Calcineurin B (aa7-37). This antibody is tested and proven to work in the following applications:

Calcineurin Subunit B Type 1 (PPP3R1) Antibody

20-abx114854
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Calcineurin Subunit B Type 1 (PPP3R1) Antibody

abx122626-100ug 100 ug
EUR 391

Calcineurin Subunit B Type 1 (PPP3R1) Antibody

20-abx000913
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
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Calcineurin Subunit B Type 1 (PPP3R1) Antibody

abx236731-100ug 100 ug
EUR 481

Calcineurin Subunit B Type 1 (PPP3R1) Antibody

20-abx302644
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
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Calcineurin Subunit B Type 1 (PPP3R1) Antibody

20-abx217914
  • EUR 425.00
  • EUR 342.00
  • 100 ug
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PPP3R1 sgRNA CRISPR Lentivector (Human) (Target 1)

K1709302 1.0 ug DNA
EUR 154

PPP3R1 sgRNA CRISPR Lentivector (Human) (Target 2)

K1709303 1.0 ug DNA
EUR 154

PPP3R1 sgRNA CRISPR Lentivector (Human) (Target 3)

K1709304 1.0 ug DNA
EUR 154

Ppp3r1 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6874002 1.0 ug DNA
EUR 154

Ppp3r1 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6874003 1.0 ug DNA
EUR 154

Ppp3r1 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6874004 1.0 ug DNA
EUR 154

PPP3R1 3'UTR GFP Stable Cell Line

TU068697 1.0 ml
EUR 1521

PPP3R1 Protein Vector (Rat) (pPB-C-His)

PV295794 500 ng
EUR 603

PPP3R1 Protein Vector (Rat) (pPB-N-His)

PV295795 500 ng
EUR 603

PPP3R1 Protein Vector (Rat) (pPM-C-HA)

PV295796 500 ng
EUR 603

PPP3R1 Protein Vector (Rat) (pPM-C-His)

PV295797 500 ng
EUR 603

PPP3R1 Protein Vector (Human) (pPB-C-His)

PV032581 500 ng
EUR 329

PPP3R1 Protein Vector (Human) (pPB-N-His)

PV032582 500 ng
EUR 329

PPP3R1 Protein Vector (Human) (pPM-C-HA)

PV032583 500 ng
EUR 329

PPP3R1 Protein Vector (Human) (pPM-C-His)

PV032584 500 ng
EUR 329

Recombinant Human PPP3R1 Protein, His, E.coli-1mg

QP13129-1mg 1mg
EUR 2757

Recombinant Human PPP3R1 Protein, His, E.coli-20ug

QP13129-20ug 20ug
EUR 201

Recombinant Human PPP3R1 Protein, His, E.coli-5ug

QP13129-5ug 5ug
EUR 155

PPP3R1 Protein Vector (Mouse) (pPB-C-His)

PV218758 500 ng
EUR 603

PPP3R1 Protein Vector (Mouse) (pPB-N-His)

PV218759 500 ng
EUR 603

PPP3R1 Protein Vector (Mouse) (pPM-C-HA)

PV218760 500 ng
EUR 603

PPP3R1 Protein Vector (Mouse) (pPM-C-His)

PV218761 500 ng
EUR 603

Ppp3r1 3'UTR Luciferase Stable Cell Line

TU216693 1.0 ml Ask for price

PPP3R1 3'UTR Luciferase Stable Cell Line

TU018697 1.0 ml
EUR 1521

Ppp3r1 3'UTR GFP Stable Cell Line

TU266693 1.0 ml Ask for price

Recombinant Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1)

4-RPE020Hu01
  • EUR 467.36
  • EUR 228.00
  • EUR 1477.60
  • EUR 559.20
  • EUR 1018.40
  • EUR 376.00
  • EUR 3544.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Protein Phosphatase 3, Regulatory Subunit 1 expressed in: E.coli

Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Antibody

20-abx129114
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Calcineurin Subunit B Type 1 (PPP3R1) Antibody (HRP)

20-abx304942
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Calcineurin Subunit B Type 1 (PPP3R1) Antibody (FITC)

20-abx304943
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Calcineurin Subunit B Type 1 (PPP3R1) Antibody (Biotin)

20-abx304944
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Antibody

20-abx178172
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Antibody

20-abx178173
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

PPP3R1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV676135 1.0 ug DNA
EUR 514

PPP3R1 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV676139 1.0 ug DNA
EUR 514

PPP3R1 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV676140 1.0 ug DNA
EUR 514

Human PPP3R1/ Calcineurin subunit B type 1 ELISA Kit

E2016Hu 1 Kit
EUR 571

Rat Ppp3r1/ Calcineurin subunit B type 1 ELISA Kit

E0789Ra 1 Kit
EUR 571

Mouse Calcineurin Subunit B Type 1 (PPP3R1) ELISA Kit

abx570694-96tests 96 tests
EUR 668

Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Protein

20-abx654868
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Protein

20-abx654869
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cow Calcineurin Subunit B Type 1 (PPP3R1) ELISA Kit

abx516389-96tests 96 tests
EUR 911

Human Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) Protein

20-abx166252
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Calcineurin Subunit B Type 1 (PPP3R1) ELISA Kit

abx250774-96tests 96 tests
EUR 668

Rat Calcineurin Subunit B Type 1 (PPP3R1) ELISA Kit

abx256480-96tests 96 tests
EUR 668

Rat Ppp3r1(Calcineurin subunit B type 1) ELISA Kit

ER0503 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Human PPP3R1(Calcineurin subunit B type 1) ELISA Kit

EH1491 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Bovine Calcineurin subunit B type 1, PPP3R1 ELISA KIT

ELI-04364b 96 Tests
EUR 928

Mouse Calcineurin subunit B type 1, Ppp3r1 ELISA KIT

ELI-04365m 96 Tests
EUR 865

Human Calcineurin subunit B type 1, PPP3R1 ELISA KIT

ELI-04367h 96 Tests
EUR 824

Rat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E02C2398-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E02C2398-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E02C2398-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Calcineurin subunit B type 1(PPP3R1) ELISA kit

E09C2398-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Calcineurin subunit B type 1(PPP3R1) ELISA kit

E09C2398-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Calcineurin subunit B type 1(PPP3R1) ELISA kit

E09C2398-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Calcineurin subunit B type 1(PPP3R1) ELISA kit

E08C2398-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Calcineurin subunit B type 1(PPP3R1) ELISA kit

E08C2398-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Calcineurin subunit B type 1(PPP3R1) ELISA kit

E08C2398-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Calcineurin subunit B type 1(PPP3R1) ELISA kit

E01C2398-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Calcineurin subunit B type 1(PPP3R1) ELISA kit

E01C2398-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Calcineurin subunit B type 1(PPP3R1) ELISA kit

E01C2398-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Calcineurin subunit B type 1(PPP3R1) ELISA kit

E04C2398-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Calcineurin subunit B type 1(PPP3R1) ELISA kit

E04C2398-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Calcineurin subunit B type 1(PPP3R1) ELISA kit

E04C2398-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Calcineurin subunit B type 1(PPP3R1) ELISA kit

E03C2398-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Calcineurin subunit B type 1(PPP3R1) ELISA kit

E03C2398-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Calcineurin subunit B type 1(PPP3R1) ELISA kit

E03C2398-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Calcineurin subunit B type 1(PPP3R1) ELISA kit

E07C2398-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Calcineurin subunit B type 1(PPP3R1) ELISA kit

E07C2398-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Calcineurin subunit B type 1(PPP3R1) ELISA kit

E07C2398-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E06C2398-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E06C2398-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Calcineurin subunit B type 1(PPP3R1) ELISA kit

E06C2398-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat Calcineurin subunit B type 1(PPP3R1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

SEE020Mu-10x96wellstestplate 10x96-wells test plate
EUR 4862.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) in Tissue homogenates and other biological fluids.

One is Phactr1, actin-binding protein that recruits protein phosphatase 1 (PP1) to specific phosphoprotein substrates. Using co-immunoprecipitation, we found that Phactr1 required to connect the channel to actin. Using a clamp recordings, we found that co-expression of wild-type channels Phactr1 with Slack reduce current amplitude but had no effect on the channel Slack where preserved PKC phosphorylation sites (S407), which regulates the current amplitude has been mutated.