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Dopamine and cAMP-regulated phosphoprotein 32kDa (DARPP-32), protein phosphatase1 and cyclin-dependent kinase 5 expression in ovarian cancer

Dopamine and cAMP-regulated phosphoprotein 32kDa (DARPP-32), protein phosphatase-1 and cyclin-dependent kinase 5 expression in ovarian cancer
Posted by Karen

Dopamine and cyclic-AMP activated Mr32kDa phosphoprotein (DARPP-32) is a central signaling proteins in neurotransmission. Here DARPP-32 phosphorylation by protein kinase A (PKA), DARPP-32 into a powerful protein phosphatase 1 (PP1) inhibitor. DARPP-32 can itself inhibit the following PKA DARPP-32 phosphorylation by cyclin-dependent kinase 5 (Cdk5). Increasing evidence suggests a role for DARPP-32 and signal pathways related to cancer;

However, its role in ovarian cancer remains unclear. Using immunohistochemistry, expression of DARPP-32, PP1 and Cdk5 is determined in a large cohort of primary tumors from ovarian cancer patients (n = 428, 445 and 434 respectively) to evaluate the relationship between clinical outcome and clinicopathological criteria.

Low cytoplasmic and nuclear DARPP-32 expression was associated with a shorter overall patient survival and progression-free survival (P = 0.001, 0.001, 0.004 and 0.037 respectively). Low nuclear and cytoplasmic DARPP-32 expression remained significantly associated with overall survival in multivariate Cox regression (P = 0.045, hazard ratio (HR) = 0.734, 95% confidence interval (CI) = 0.542 to 0.993 and P = 0.001, HR = 0.494, 95% CI = 0.325 to 0.749, respectively).

cytoplasmic and nuclear high PP1 expression was associated with a shorter overall patient survival and expression of cytoplasmic PP1 high with progression-free survival shorter (P = 0.005, 0.033, and 0.037, respectively). Cdk5 high expression was associated with progression-free survival shorter (P = 0.006). These data demonstrate the role of DARPP-32 and related kinase signaling as a prognostic marker in ovarian cancer clinical utility.

Dopamine and cAMP-regulated phospho<em>protein</em> 32kDa (DARPP-32), <em>protein</em> <em>phosphatase</em>-<em>1</em> and cyclin-dependent kinase 5 expression in ovarian cancer
Dopamine and cAMP-regulated phosphoprotein 32kDa (DARPP-32), protein phosphatase1 and cyclin-dependent kinase 5 expression in ovarian cancer

Protein phosphatase one alpha Increase glucocorticoid receptor activity by a mechanism that involves phosphorylation of serine-2 one one < / em>

By acting as a ligand-dependent transcription factor of the glucocorticoid receptor (GR) mediates the action of glucocorticoids and regulates many physiological processes. A disturbed regulation of glucocorticoid action has been associated with various disorders. Thus, an explanation of the underlying signaling pathway is important for understanding the mechanisms of glucocorticoid impaired function and contribute to disease.

This study found an increase in excess GR transcriptional activity of protein phosphatase 1 alpha (PP1α) in HEK-293 cells and a decrease in the expression levels of GR-responsive gene knockdown following PP1α model A549 cells endogenously. Mechanistic investigations revealed reduce GR-Ser211 phosphorylation following PP1α silencing and provide the first indication of the involvement of glycogen synthase kinase 3 (GSK-3).

Thus, this study identified PP1α as a novel post-translational activator of GR signaling, indicating that PP1α function disorders can lead to disorders glucocorticoid action and thereby contribute to diseases.Ewing sarcoma (ES) is a pediatric malignant bone and soft tissue tumors. Patients with metastatic ES have dismal outcomes were not improved in decades. The major challenge in the treatment of metastatic ES is the lack of specific targets and rational combination therapy.

PPP1CA siRNA

20-abx904171
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

PPP1CA Antibody

CSB-PA018503KA01HU-
EUR 335
Description: A polyclonal antibody against PPP1CA. Recognizes PPP1CA from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

PPP1CA Antibody

CSB-PA018503KA01HU-100ul 100ul
EUR 389
Description: A polyclonal antibody against PPP1CA. Recognizes PPP1CA from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

PPP1CA Antibody

1-CSB-PA018503LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP1CA. Recognizes PPP1CA from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

PPP1CA Antibody

1-CSB-PA030161
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP1CA. Recognizes PPP1CA from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000

PPP1CA Antibody

BF0683 200ul
EUR 376
Description: PPP1CA antibody detects endogenous levels of total PPP1CA.

PPP1CA Antibody

DF6907 200ul
EUR 304
Description: PPP1CA Antibody detects endogenous levels of total PPP1CA.

PPP1CA Antibody

ABD6907 100 ug
EUR 438

PPP1CA antibody

10R-1069 100 ul
EUR 316
Description: Mouse monoclonal PPP1CA antibody

PPP1CA antibody

70R-2013 50 ug
EUR 467
Description: Rabbit polyclonal PPP1CA antibody raised against the N terminal of PPP1CA

PPP1CA Protein (Recombinant)

20-abx073956
  • EUR 230.00
  • EUR 1609.00
  • EUR 328.00
  • 1 µg
  • 50 ug
  • 5 ug

PPP1CA Rabbit pAb

A12468-100ul 100 ul
EUR 308

PPP1CA Rabbit pAb

A12468-200ul 200 ul
EUR 459

PPP1CA Rabbit pAb

A12468-20ul 20 ul
EUR 183

PPP1CA Rabbit pAb

A12468-50ul 50 ul
EUR 223

PPP1CA + 1CB Antibody

20-abx217900
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Anti-PPP1CA antibody

STJ25080 100 µl
EUR 277
Description: The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

PPP1CA Monoclonal Antibody

27087-100ul 100ul
EUR 252

PPP1CA Monoclonal Antibody

27087-50ul 50ul
EUR 187

Anti-PPP1CA antibody

STJ114342 100 µl
EUR 277
Description: The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

PPP1CA cloning plasmid

CSB-CL018503HU-10ug 10ug
EUR 233
Description: A cloning plasmid for the PPP1CA gene.

PPP1CA Conjugated Antibody

C32649 100ul
EUR 397

PPP1CA + 1CB Antibody

45032-100ul 100ul
EUR 252

PPP1CA + 1CB Antibody

45032-50ul 50ul
EUR 187

PPP1CA + 1CB Antibody

DF2954 200ul
EUR 304
Description: PPP1CA + 1CB Antibody detects endogenous levels of total PPP1CA + 1CB.

PPP1CA Blocking Peptide

BF0683-BP 1mg
EUR 195

anti-PPP1CA (5E9)

LF-MA30669 100 ul
EUR 527
Description: Mouse Monoclonal to PPP1CA

PPP1CA Blocking Peptide

DF6907-BP 1mg
EUR 195

PPP1CA + 1CB Antibody

ABD2954 100 ug
EUR 438

Human Recombinant PPP1CA

6370-10
EUR 403

PPP1CA Blocking Peptide

33R-6411 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of PPP1CA antibody, catalog no. 70R-2013

Rat PPP1CA shRNA Plasmid

20-abx984590
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human PPP1CA shRNA Plasmid

20-abx953684
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse PPP1CA shRNA Plasmid

20-abx972168
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

PPP1CA + 1CB Conjugated Antibody

C45032 100ul
EUR 397

PPP1CA Antibody, HRP conjugated

1-CSB-PA018503LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP1CA. Recognizes PPP1CA from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

PPP1CA Antibody, FITC conjugated

1-CSB-PA018503LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP1CA. Recognizes PPP1CA from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

PPP1CA Antibody, Biotin conjugated

1-CSB-PA018503LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP1CA. Recognizes PPP1CA from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Phospho-PPP1CA (T320) Antibody

1-CSB-PA030160
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Phospho-PPP1CA (T320). Recognizes Phospho-PPP1CA (T320) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/10000

PPP1CA Conjugated Monoclonal Antibody

C27087 100ul
EUR 397

PPP1CA + 1CB Blocking Peptide

DF2954-BP 1mg
EUR 195

Mouse Ppp1ca ELISA KIT

ELI-36183m 96 Tests
EUR 865

Bovine PPP1CA ELISA KIT

ELI-45373b 96 Tests
EUR 928

Human PPP1CA ELISA KIT

ELI-16516h 96 Tests
EUR 824

Canine PPP1CA ELISA KIT

ELI-21782d 96 Tests
EUR 928

Human PPP1CA ELISA Kit

ELA-E9568h 96 Tests
EUR 824

PPP1CA ELISA KIT|Human

EF006522 96 Tests
EUR 689

PPP1CA Recombinant Protein (Mouse)

RP163841 100 ug Ask for price

PPP1CA Recombinant Protein (Human)

RP024331 100 ug Ask for price

PPP1CA Recombinant Protein (Rat)

RP221681 100 ug Ask for price

PPP1CA protein (His tag)

80R-1935 10 ug
EUR 322
Description: Recombinant human PPP1CA protein

Phospho-PPP1CA-T320 Rabbit pAb

AP0786-100ul 100 ul
EUR 384

Phospho-PPP1CA-T320 Rabbit pAb

AP0786-200ul 200 ul
EUR 554

Phospho-PPP1CA-T320 Rabbit pAb

AP0786-20ul 20 ul
EUR 183

Phospho-PPP1CA-T320 Rabbit pAb

AP0786-50ul 50 ul
EUR 265

Monoclonal PPP1CA Antibody, Clone: 5E9

AMM02873G 0.1ml
EUR 528
Description: A Monoclonal antibody against Human PPP1CA. The antibodies are raised in Mouse and are from clone 5E9. This antibody is applicable in WB, E

PPP1CA ELISA Kit (Mouse) (OKEH04977)

OKEH04977 96 Wells
EUR 727
Description: Description of target: Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates CENPA. Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.17 ng/mL

PPP1CA ELISA Kit (Human) (OKEH01979)

OKEH01979 96 Wells
EUR 727
Description: Description of target: The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.055 ng/mL

Anti-Phospho-PPP1CA-(T320) antibody

STJ117884 100 µl
EUR 393
Description: The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

PPP1CA ELISA Kit (Rat) (OKEH05943)

OKEH05943 96 Wells
EUR 727
Description: Description of target: Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates CENPA. Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.171 ng/mL

PPP1CA ELISA Kit (Bovine) (OKEH06691)

OKEH06691 96 Wells
EUR 779
Description: Description of target: Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates CENPA. Dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy.;Species reactivity: Bovine;Application: ;Assay info: Assay Methodology: Quantitative Competitive ELISA;Sensitivity: 0.25 ng/mL

PPP1CA ELISA Kit (Dog) (OKEH08782)

OKEH08782 96 Wells
EUR 1184
Description: Description of target: ;Species reactivity: Dog;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity:

PPP1CA ORF Vector (Human) (pORF)

ORF008111 1.0 ug DNA
EUR 95

Ppp1ca ORF Vector (Mouse) (pORF)

ORF054615 1.0 ug DNA
EUR 506

Ppp1ca ORF Vector (Rat) (pORF)

ORF073895 1.0 ug DNA
EUR 506

Anti-PPP1CA+PPP1CB Rabbit Monoclonal Antibody

M02801 100ug/vial
EUR 397
Description: Rabbit Monoclonal PPP1CA+PPP1CB Antibody. Validated in IP, IF, IHC, WB and tested in Human, Mouse, Rat.

Ppp1ca sgRNA CRISPR Lentivector set (Mouse)

K3345601 3 x 1.0 ug
EUR 339

PPP1CA sgRNA CRISPR Lentivector set (Human)

K1701701 3 x 1.0 ug
EUR 339

Ppp1ca sgRNA CRISPR Lentivector set (Rat)

K7563101 3 x 1.0 ug
EUR 339

Phospho-PPP1CA-T320 Antibody Kit (AP0786 & A12468)

RK04085 50 ul
EUR 344

Ppp1ca sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3345602 1.0 ug DNA
EUR 154

Ppp1ca sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3345603 1.0 ug DNA
EUR 154

Ppp1ca sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3345604 1.0 ug DNA
EUR 154

PPP1CA sgRNA CRISPR Lentivector (Human) (Target 1)

K1701702 1.0 ug DNA
EUR 154

PPP1CA sgRNA CRISPR Lentivector (Human) (Target 2)

K1701703 1.0 ug DNA
EUR 154

PPP1CA sgRNA CRISPR Lentivector (Human) (Target 3)

K1701704 1.0 ug DNA
EUR 154

Ppp1ca sgRNA CRISPR Lentivector (Rat) (Target 1)

K7563102 1.0 ug DNA
EUR 154

Ppp1ca sgRNA CRISPR Lentivector (Rat) (Target 2)

K7563103 1.0 ug DNA
EUR 154

Ppp1ca sgRNA CRISPR Lentivector (Rat) (Target 3)

K7563104 1.0 ug DNA
EUR 154

Ppp1ca 3'UTR GFP Stable Cell Line

TU266644 1.0 ml Ask for price

Ppp1ca 3'UTR GFP Stable Cell Line

TU166799 1.0 ml Ask for price

Ppp1ca 3'UTR Luciferase Stable Cell Line

TU216644 1.0 ml Ask for price

Ppp1ca 3'UTR Luciferase Stable Cell Line

TU116799 1.0 ml Ask for price

PPP1CA 3'UTR Luciferase Stable Cell Line

TU018618 1.0 ml
EUR 1394

PPP1CA 3'UTR GFP Stable Cell Line

TU068618 1.0 ml
EUR 1394

PPP1CA Protein Vector (Human) (pPB-C-His)

PV032441 500 ng
EUR 329

PPP1CA Protein Vector (Human) (pPB-N-His)

PV032442 500 ng
EUR 329

PPP1CA Protein Vector (Human) (pPM-C-HA)

PV032443 500 ng
EUR 329

PPP1CA Protein Vector (Human) (pPM-C-His)

PV032444 500 ng
EUR 329

PPP1CA Protein Vector (Mouse) (pPB-C-His)

PV218458 500 ng
EUR 603

PPP1CA Protein Vector (Mouse) (pPB-N-His)

PV218459 500 ng
EUR 603

PPP1CA Protein Vector (Mouse) (pPM-C-HA)

PV218460 500 ng
EUR 603

PPP1CA Protein Vector (Mouse) (pPM-C-His)

PV218461 500 ng
EUR 603

PPP1CA Protein Vector (Rat) (pPB-C-His)

PV295578 500 ng
EUR 603

PPP1CA Protein Vector (Rat) (pPB-N-His)

PV295579 500 ng
EUR 603

PPP1CA Protein Vector (Rat) (pPM-C-HA)

PV295580 500 ng
EUR 603

We recently discovered a protein phosphatase 1 regulatory subunit 1A (PPP1R1A) specifically highly expressed in ES and promote tumor growth and metastasis in ES. In the current investigation, we showed that PPP1R1A regulate ES cell growth cycle at the G1 / S phase to down-regulate the cell cycle inhibitor p21Cip1 and p27KIP1, which leads to retinoblastoma (Rb) protein hyperphosphorylation. In addition, we show that the normal transcription PPP1R1A promotes histone genes during cell cycle progression.

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