Bladder cancer (BC) is the ninth most common tumors in the world and one of the most common malignant tumor of the urinary system. Several studies have reported that the expression of miR-133b reduced in BC, but whether it plays a role in the development of BC and the mechanism is unclear. microRNAs can be packaged into exosomes to mediate communication between tumor cells, affecting their proliferation and apoptosis.
The purpose of this study was to determine the effect of miR-133b exosomal in BC proliferation and its molecular mechanism. First, the expression of miR-133b was evaluated in BC and adjacent normal tissue, as well as in the patient’s serum exosomes BC and healthy controls. Then the delivery and internalization of exosomes in cell localization was observed through fluorescence. cell viability and apoptosis assessed in cells transfected with imitate BC and incubated with exosomes.
Exosomal the role of miR-133b is also analyzed in nude mice transplanted tumor. Furthermore, miR-133b target genes predicted through bioinformatics. Levels of miR-133b was significantly decreased in the SM network and in exosomes from serum of patients, which correlated with poor overall survival in TCGA. Exosomal miR-133b can be obtained using the cells BC after transfection with miR-133b mimic.
Expression of miR-133b increased after incubation with exosomal miR-133b, which causes inhibition of viability and increased cell apoptosis in BC. Exosomal miR-133b can suppress tumor growth in vivo. In addition, we found that miR-133b exosomal may play a role in suppressing the proliferation by upregulating SM dual-specificity protein phosphatase 1 (DUSP1). These findings may offer promise for new therapeutic directions BC.
Exosome-transmitted microRNA-133b inhibited bladder cancer proliferation by upregulating dual-specificity proteinphosphatase1
Protein phosphatase one 35 regulatory subunit required for ciliogenesis, morphogenesis notochord, and the development of cell-cycle during the development of murine
protein phosphatases regulate a variety of proteins with post-translational modifications and necessary for most intracellular events in eukaryotes. While some of the core components of complex protein phosphatase marked, many large complex subunit remained unstudied. Here we characterize the allele loss-of-function protein phosphatase 1 regulatory subunit 35 (Ppp1r35) gene.
Ppp1r35 homozygous mouse embryos lacking is the development of a delayed start at embryonic day (E) 7.5 and have morphological defects obvious at later stages. Mutant fails to start rotating and not advancing beyond the size or staging normal E8.5 embryos. Consistent with the latest in in vitro studies linking PPP1R35 with microcephaly protein Rotatin and with roles in centrosome formation, we showed that the mutant embryos Ppp1r35 shortage of primary cilia.
Histological and molecular analysis of mutants revealed that the development of the notochord Ppp1r35 irregular and discontinuous, and is consistent with a role in primary cilia, that the floor plate of the neural tube is not specified. Similar to other mutant embryos with defects in the function of centrioles, Ppp1r35 mutant displayed increased cell death that is prevalent in the neural tube and an increasing number of proliferative cells in prometaphase.
Description: A polyclonal antibody against ALPPL2. Recognizes ALPPL2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100
Description: A polyclonal antibody against ALPP/ALPPL2. Recognizes ALPP/ALPPL2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000
Description: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase.
Description: A polyclonal antibody for detection of ALPP/ALPPL2 from Human, Mouse. This ALPP/ALPPL2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALPP/ALPPL2
Description: A polyclonal antibody for detection of ALPP/ALPPL2 from Human, Mouse. This ALPP/ALPPL2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALPP/ALPPL2
Description: A polyclonal antibody for detection of ALPP/ALPPL2 from Human, Mouse. This ALPP/ALPPL2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human ALPP/ALPPL2
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ALPPL2 (N-term). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human ALPPL2 - N-terminal region. This antibody is tested and proven to work in the following applications:
Description: A Monoclonal antibody against Human ALPPL2 (monoclonal) (M07). The antibodies are raised in Mouse and are from clone 2B3. This antibody is applicable in WB and IHC, IP
Description: Quantitativesandwich ELISA kit for measuring Human Alkaline phosphatase, placental-like (ALPPL2) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human Alkaline phosphatase, placental-like(ALPPL2) ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Human Alkaline phosphatase, placental-like(ALPPL2) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Rat Alkaline phosphatase, placental like(ALPPL2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Alkaline phosphatase, placental like(ALPPL2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Alkaline phosphatase, placental like(ALPPL2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Alkaline phosphatase, placental like(ALPPL2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Alkaline phosphatase, placental like(ALPPL2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Alkaline phosphatase, placental like(ALPPL2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Alkaline phosphatase, placental like(ALPPL2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Alkaline phosphatase, placental like(ALPPL2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Alkaline phosphatase, placental like(ALPPL2) ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Alkaline phosphatase, placental- like, ALPPL2 ELISA KIT
Description: A competitive ELISA for quantitative measurement of Guinea pig Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Guinea pig Alkaline phosphatase, placental like(ALPPL2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) in Tissue homogenates and other biological fluids.
Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) in Tissue homogenates and other biological fluids.
Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) in Tissue homogenates and other biological fluids.
Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) in Tissue homogenates and other biological fluids.
Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
ELISA kit for Human ALPPL2 (Alkaline Phosphatase, Placental Like Protein 2)
Description: A sandwich ELISA kit for detection of Alkaline Phosphatase, Placental Like Protein 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A Rabbit polyclonal antibody against Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2). This antibody is labeled with FITC.
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We hypothesize that the loss of homeostasis function abrogates Ppp1r35 centrioles, which resulted in failure to yield the major functional cilia, cell death and cell cycle delay / stalling that leads to the failure of development. Taken together, these results highlight the important function of Ppp1r35 during early mammalian development and involving this gene as a candidate for human microcephaly.
