hepatitis B virus (HBV) DNA replicates its genome through DNA virus prime reverse transcription polymerase themselves of pre-genomic RNA in the nucleocapsid core protein is assembled by 120 (Cp) dimer. The carboxyl-terminal arginine-rich domain (CTD) Cp play an important role in selective packaging of viral DNA polymerase-pregenomic (pg) RNA into the nucleocapsid complex. Earlier research has suggested that CTD initially phosphorylated at multiple sites to facilitate the packaging of viral RNA and then dephosphorylated with regard to viral DNA synthesis and secretion of DNA containing virions.
However, our recent studies suggest that Cp is hyper-phosphorylated as a free dimer and dephosphorylation associated with pgRNA encapsidation. Here, we provide genetic and biochemical evidence of more extensive supporting Cp dephosphorylation not take place during the assembly pgRNA containing the nucleocapsid, but not empty capsids. In addition, we found that the cellular protein phosphatase 1 (PP1) is necessary for dephosphorylation and packaging pgRNA Cp.
Interestingly, PP1 catalytic subunit α and β are packed into pgRNA containing the nucleocapsid, but not empty capsids, and the treatment of HBV-replicating cells with core protein allosteric modulator (CpAMs) promoted empty capsid assembly and encapsidation repeal of PP1 α and β. Our study thus identified PP1 as the host cellular factors that are co-packaged into the nucleocapsid HBV, and plays an important role in selective packaging of viral DNA-polymerase-catalyzed pgRNA complex via Cp dephosphorylation.
Proteinphosphatase1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids
A novel protein tyrosine phosphatase one B inhibitor-geranylated flavonoids from mulberry leaves ameliorates insulin resistance
Mulberry leaf vegetables are common to a variety of beneficial effects, such as hypoglycemic activity. However, the underlying mechanism of the hypoglycemic effect that has not been revealed fully. In this study, two flavonoid derivatives isolated from mulberry leaves, a new geranylated flavonoid compounds (1) and a structural analogue (2). Structures 1 and 2 are described using spectroscopic analysis.
To study the properties of the hypoglycemic potential of this compound, the effect of the regulation of protein tyrosine phosphatase 1B (PTP1B) was investigated. Compared with oleanolic acid, compounds 1 and 2 showed a significant inhibitory activity (IC50 = 4.53 ± 0.31 and 10.53 ± 1.76 M) against PTP1B, a positive control (IC50 = 7.94 ± 0.76 M). molecular docking to predict the binding sites 1 and PTP1B.
In insulin resistance HepG2 cells, first promoted glucose consumption with a dose-dependent manner. Furthermore, western blot and PCR analysis showed that one may regulate the consumption of glucose by PTP1B / IRS / PI3K / AKT pathways. In conclusion, the mulberry leaf flavonoids inhibite geranylated PTP1B and increase the consumption of glucose in insulin-resistant cells. These findings provide an important basis for the use of mulberry leaf flavonoids as a dietary supplement to regulate glucose metabolism.
Description: A polyclonal antibody against PTPRC. Recognizes PTPRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:5000, IHC:1:50-1:200
Description: A polyclonal antibody against PTPRC. Recognizes PTPRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:10000, IHC:1:100-1:300
Description: A polyclonal antibody against PTPRC. Recognizes PTPRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000
Description: A polyclonal antibody against PTPRC. Recognizes PTPRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200
Description: A polyclonal antibody against PTPRC. Recognizes PTPRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200
Description: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Description: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Description: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Description: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Description: CD45 is a receptor protein tyrosine phosphatase, also known as Ly-5 or leukocyte common antigen. CD45 mainly involves in the initiation of T cell receptor signaling by controlling the activation of the Src family protein-tyrosine kinases Lck and Fyn. CD45 deficiency causes in T- and B-lymphocyte dysfunction in the form of severe combined immune deficiency. It also takes a significant role in autoimmune diseases and cancer as well as in infectious diseases including fungal infections.
Description: A polyclonal antibody against Phospho-PTPRC (Ser1007). Recognizes Phospho-PTPRC (Ser1007) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000
Description: Primary antibody against CD45RA(PTPRC/1148), CF660R conjugate, Concentration: 0.1mg/mL
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ten different cDNA encoding five different protein phosphatase 1 (ppp1) were cloned from Nilaparvata lugens. NlPPP1α and NlPPP1β highly conserved while NlPPP1-Y, NlPPP1-Y1 and Y2 NlPPP1 low-conserved between insects. NlPPP1α and NlPPP1β exhibited expression everywhere, while NlPPP1-Y, NlPPP1-Y1, and NlPPP1-Y2 are clearly detectable from instar nymphs 4 to adult stage of development in males, mainly detected in the internal reproductive organs and body fat than men -laki. nymphs injection with dsRNA of NlPPP1α or NlPPP1β able to reduce the expression of target genes in the range of 71.5 to 91.0%, pushing the maximum mortality rate of 95.2% or 97.2% on day 10 after the injection and eclosion rate fell 65, 5 to 100.0%.
