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SIRT2 is involved in cisplatin-induced acute kidney injury through regulation of mitogen-activated protein kinase phosphatase1.

SIRT2 is involved in cisplatin-induced acute kidney injury through regulation of mitogen-activated protein kinase phosphatase-1.
Posted by Karen

Activation of mitogen-activated protein kinase phosphatase-1 (MKP-1), dual-specificity protein phosphatase, set the mitogen-activated protein kinase signaling. C-Jun N-terminal kinase (JNK) and p38 activated cisplatin-induced renal injury. However, changes in the expression of MKP-1 cisplatin-induced kidney injury and the effects of regulation sirtuin 2 (SIRT2), a nicotinamide adenine dinucleotide dependent deacetylase, the MKP-1 remains unknown.

To solve this problem, we use the constitution Sirt2 knockout (KO) mice, transgenic (TG) mice with increased expression of SIRT2 particularly in the proximal tubule epithelial cellsand wild-type (WT) mice. Cisplatin nephrotoxicity induced by intraperitoneal injection of cisplatin.MKP-1 expression in the kidney to decline after cisplatin treatment. Cisplatin-induced downregulation of MKP-1 KO mice Sirt2 reversed in the kidneys and then decreased in TG mice Sirt2 kidney.

We observed a similar phenomenon with SIRT2-knockdown or SIRT2-expressed in tubular epithelial cells. Phosphorylation of p38 and JNK, downstream signaling pathways MKP-1, is increased in WT mice after treatment with cisplatin kidney. Decrease SIRT2 suppressed cisplatin-induced phosphorylation of p38 and JNK in renal and tubular epithelial cells.

Excess of SIRT2 increase phosphorylation of p38 and JNK in renal and tubular epithelial cells. Acetylation of MKP-1 increased significantly in SIRT2-knockdown cells and a decrease in SIRT2-expressed cells after stimulation cisplatin. Sirt2 KO mice and Sirt2 TG mice showed improvement and aggravation of kidney injury, apoptosis, necroptosis and inflammation caused by cisplatin.Our data showed that SIRT2 associated with cisplatin-induced renal injury through regulation MKP-1 expression.

SIRT2 is involved in cisplatin-induced acute kidney injury through regulation of mitogen-activated <em>protein</em> kinase <em>phosphatase</em>-<em>1</em>.
SIRT2 is involved in cisplatin-induced acute kidney injury through regulation of mitogen-activated protein kinase phosphatase1.

Protein phosphatase one controls the activity of a balance between collective and singular mode of cell migration.

collective cell migration is central to many developmental and pathological processes. However, the collective mechanisms that keep cells together and coordinate the movement of some cells are poorly understood. Using a Drosophila Model border cell migration, we found that cell cohesion protein phosphatase 1 (PP1) activity collective control and migration.

Inhibition of PP1 causes cells to get round the border, which split off, and move it as single cell motility changes. We are proof that PP1 promoting appropriate levels of cadherin-catenin protein-complex at the intersection of the cells in the cluster to keep the cells along the border. Limiting PP1 actomyosin contractility advanced to the outskirts of the cluster rather than on the internal border cell contact that individual. We showed that the myosin phosphatase PP1 complex, which blocks non-muscle myosin II- (Myo-II) activity, cell shape and the boundary coordinates of cluster cohesion.

Given the high conservation of the PP1 complex, this study identifies collective PP1 as the main regulator of the single cell migration.
Protein phosphatase methylesterase 1 has been identified as a new gene that is upregulated in skeletal muscle in response to neurogenic atrophy in mice. Western blot analysis confirms that Ppme1 expressed during both muscle cell proliferation and differentiation.

PPP1R15A siRNA

20-abx929482
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

PPP1R15A siRNA

20-abx904181
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

PPP1R15A antibody

10R-7104 100 ul
EUR 829.2
Description: Mouse monoclonal PPP1R15A antibody

PPP1R15A antibody

10R-7105 100 ul
EUR 829.2
Description: Mouse monoclonal PPP1R15A antibody

PPP1R15A antibody

10R-7106 100 ul
EUR 829.2
Description: Mouse monoclonal PPP1R15A antibody

PPP1R15A Antibody

39948-100ul 100ul
EUR 468

PPP1R15A Antibody

43273-100ul 100ul
EUR 302.4

PPP1R15A Antibody

1-CSB-PA787946
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP1R15A. Recognizes PPP1R15A from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-2000, ELISA:1:10000-20000

PPP1R15A Antibody

1-CSB-PA018528GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against PPP1R15A. Recognizes PPP1R15A from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB

PPP1R15A Rabbit pAb

A16260-100ul 100 ul
EUR 369.6

PPP1R15A Rabbit pAb

A16260-200ul 200 ul
EUR 550.8

PPP1R15A Rabbit pAb

A16260-20ul 20 ul
EUR 219.6

PPP1R15A Rabbit pAb

A16260-50ul 50 ul
EUR 267.6

PPP1R15A cloning plasmid

CSB-CL018528HU-10ug 10ug
EUR 812.4
Description: A cloning plasmid for the PPP1R15A gene.

Ppp1r15a Antibody / Gadd34

RQ7322 100ug
EUR 419

PPP1R15A Conjugated Antibody

C43273 100ul
EUR 476.4

Human PPP1R15A ELISA KIT

ELI-43112h 96 Tests
EUR 988.8

Mouse Ppp1r15a ELISA KIT

ELI-43113m 96 Tests
EUR 1038

Bovine PPP1R15A ELISA KIT

ELI-19724b 96 Tests
EUR 1113.6

Rat PPP1R15A shRNA Plasmid

20-abx987671
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Human PPP1R15A shRNA Plasmid

20-abx958419
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse PPP1R15A shRNA Plasmid

20-abx971639
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

PPP1R15A Recombinant Protein (Rat)

RP221720 100 ug Ask for price

PPP1R15A Recombinant Protein (Mouse)

RP163886 100 ug Ask for price

PPP1R15A Recombinant Protein (Human)

RP024352 100 ug Ask for price

Ppp1r15a ORF Vector (Rat) (pORF)

ORF073908 1.0 ug DNA
EUR 607.2

PPP1R15A ORF Vector (Human) (pORF)

ORF008118 1.0 ug DNA
EUR 114

Ppp1r15a ORF Vector (Mouse) (pORF)

ORF054630 1.0 ug DNA
EUR 607.2

PPP1R15A ELISA Kit (Rat) (OKCD00864)

OKCD00864 96 Wells
EUR 1075.2
Description: Description of target: Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.1 Publication <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.6"Potential molecular mechanism for rodent tumorigenesis: mutational generation of Progression Elevated Gene-3 (PEG-3)."_x005F_x005F_x000D_Su Z.-Z., Emdad L., Sarkar D., Randolph A., Valerie K., Yacoub A., Dent P., Fisher P.B._x005F_x005F_x000D_Oncogene 24:2247-2255(2005) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, MUTANT PEG-3. ;Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.118 ng/mL

PPP1R15A ELISA Kit (Mouse) (OKCD00311)

OKCD00311 96 Wells
EUR 1028.4
Description: Description of target: Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. In case of infection with vesicular stomatitis virus (VSV), impairs viral replication.5 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.6"Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha."_x005F_x005F_x000D_Novoa I., Zeng H., Harding H.P., Ron D._x005F_x005F_x000D_J. Cell Biol. 153:1011-1022(2001) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH PP1, MUTAGENESIS OF VAL-549.Ref.8"Stress-induced gene expression requires programmed recovery from translational repression."_x005F_x005F_x000D_Novoa I., Zhang Y., Zeng H., Jungreis R., Harding H.P., Ron D._x005F_x005F_x000D_EMBO J. 22:1180-1187(2003) [PubMed] [Europe PMC] [Abstract]Cited for: INDUCTION, FUNCTION.Ref.9"The function of GADD34 is a recovery from a shutoff of protein synthesis induced by ER stress: elucidation by GADD34-deficient mice."_x005F_x005F_x000D_Kojima E., Takeuchi A., Haneda M., Yagi A., Hasegawa T., Yamaki K., Takeda K., Akira S., Shimokata K., Isobe K._x005F_x005F_x000D_FASEB J. 17:1573-1575(2003) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.Ref.10"Gadd34 requirement for normal hemoglobin synthesis."_x005F_x005F_x000D_Patterson A.D., Hollander M.C., Miller G.F., Fornace A.J. Jr._x005F_x005F_x000D_Mol. Cell. Biol. 26:1644-1653(2006) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, DISRUPTION PHENOTYPE.Ref.11"Suppression of viral replication by stress-inducible GADD34 protein via the mammalian serine/threonine protein kinase mTOR pathway."_x005F_x005F_x000D_Minami K., Tambe Y., Watanabe R., Isono T., Haneda M., Isobe K., Kobayashi T., Hino O., Okabe H., Chano T., Inoue H._x005F_x005F_x000D_J. Virol. 81:11106-11115(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INDUCTION. ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.057 ng/mL

h PPP1R15A inducible lentiviral particles

LVP854 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for expressing human target: h PPP1R15A (protein phosphatase 1, regulatory subunit 15A), [alternative names: GADD34]. The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_014330.3 . It also contains a RFP-Blasticidin dual selection marker.

Ppp1r15a 3'UTR GFP Stable Cell Line

TU166813 1.0 ml Ask for price

PPP1R15A 3'UTR GFP Stable Cell Line

TU068671 1.0 ml
EUR 1672.8

Ppp1r15a 3'UTR GFP Stable Cell Line

TU266657 1.0 ml Ask for price

Ppp1r15a sgRNA CRISPR Lentivector set (Rat)

K6981701 3 x 1.0 ug
EUR 406.8

PPP1R15A sgRNA CRISPR Lentivector set (Human)

K1706801 3 x 1.0 ug
EUR 406.8

Ppp1r15a sgRNA CRISPR Lentivector set (Mouse)

K3806501 3 x 1.0 ug
EUR 406.8

PPP1R15A Protein Vector (Rat) (pPM-C-HA)

PV295632 500 ng
EUR 723.6

PPP1R15A Protein Vector (Rat) (pPB-C-His)

PV295630 500 ng
EUR 723.6

PPP1R15A Protein Vector (Rat) (pPB-N-His)

PV295631 500 ng
EUR 723.6

PPP1R15A Protein Vector (Rat) (pPM-C-His)

PV295633 500 ng
EUR 723.6

PPP1R15A Protein Vector (Human) (pPM-C-HA)

PV032471 500 ng
EUR 394.8

PPP1R15A Protein Vector (Mouse) (pPM-C-HA)

PV218520 500 ng
EUR 723.6

PPP1R15A Protein Vector (Human) (pPB-C-His)

PV032469 500 ng
EUR 394.8

PPP1R15A Protein Vector (Human) (pPB-N-His)

PV032470 500 ng
EUR 394.8

PPP1R15A Protein Vector (Human) (pPM-C-His)

PV032472 500 ng
EUR 394.8

PPP1R15A Protein Vector (Mouse) (pPB-C-His)

PV218518 500 ng
EUR 723.6

PPP1R15A Protein Vector (Mouse) (pPB-N-His)

PV218519 500 ng
EUR 723.6

PPP1R15A Protein Vector (Mouse) (pPM-C-His)

PV218521 500 ng
EUR 723.6

Ppp1r15a 3'UTR Luciferase Stable Cell Line

TU116813 1.0 ml Ask for price

PPP1R15A 3'UTR Luciferase Stable Cell Line

TU018671 1.0 ml
EUR 1672.8

Ppp1r15a 3'UTR Luciferase Stable Cell Line

TU216657 1.0 ml Ask for price

Ppp1r15a sgRNA CRISPR Lentivector (Rat) (Target 1)

K6981702 1.0 ug DNA
EUR 184.8

Ppp1r15a sgRNA CRISPR Lentivector (Rat) (Target 2)

K6981703 1.0 ug DNA
EUR 184.8

Ppp1r15a sgRNA CRISPR Lentivector (Rat) (Target 3)

K6981704 1.0 ug DNA
EUR 184.8

PPP1R15A sgRNA CRISPR Lentivector (Human) (Target 1)

K1706802 1.0 ug DNA
EUR 184.8

PPP1R15A sgRNA CRISPR Lentivector (Human) (Target 2)

K1706803 1.0 ug DNA
EUR 184.8

PPP1R15A sgRNA CRISPR Lentivector (Human) (Target 3)

K1706804 1.0 ug DNA
EUR 184.8

Ppp1r15a sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3806502 1.0 ug DNA
EUR 184.8

Ppp1r15a sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3806503 1.0 ug DNA
EUR 184.8

Ppp1r15a sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3806504 1.0 ug DNA
EUR 184.8

PPP1R15A Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV654781 1.0 ug DNA
EUR 818.4

PPP1R15A Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV654785 1.0 ug DNA
EUR 818.4

PPP1R15A Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV654786 1.0 ug DNA
EUR 818.4

Human Protein phosphatase 1 regulatory subunit 15A (PPP1R15A)

1-CSB-YP018528HU
  • EUR 703.20
  • EUR 358.80
  • EUR 2606.40
  • EUR 1080.00
  • EUR 1730.40
  • EUR 458.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Protein phosphatase 1 regulatory subunit 15A(PPP1R15A),partial expressed in Yeast

Protein Phosphatase 1 Regulatory Subunit 15A (PPP1R15A) Antibody

abx233293-100ug 100 ug
EUR 610.8

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Antibody

20-abx325005
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Protein Phosphatase 1 Regulatory Subunit 15A (PPP1R15A) Antibody

abx412519-01mg 0.1 mg
EUR 961.2

Protein Phosphatase 1, Regulatory Subunit 15A (Ppp1r15a) Antibody

20-abx114830
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Antibody

abx036158-100ug 100 ug
EUR 469.2

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Antibody

20-abx131495
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Antibody

20-abx131496
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Antibody

abx430859-200ul 200 ul
EUR 460.8

Recombinant Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A)

4-RPC199Mu01
  • EUR 485.57
  • EUR 253.20
  • EUR 1490.88
  • EUR 576.96
  • EUR 1033.92
  • EUR 400.80
  • EUR 3547.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Protein Phosphatase 1, Regulatory Subunit 15A expressed in: E.coli

Recombinant Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A)

4-RPC199Ra01
  • EUR 485.57
  • EUR 253.20
  • EUR 1490.88
  • EUR 576.96
  • EUR 1033.92
  • EUR 400.80
  • EUR 3547.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Protein Phosphatase 1, Regulatory Subunit 15A expressed in: E.coli

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6981705 3 x 1.0 ug
EUR 451.2

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Protein

20-abx168835
  • EUR 693.60
  • EUR 309.60
  • EUR 2013.60
  • EUR 811.20
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

PPP1R15A sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K1706805 3 x 1.0 ug
EUR 451.2

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3806505 3 x 1.0 ug
EUR 451.2

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Protein

20-abx168834
  • EUR 693.60
  • EUR 309.60
  • EUR 2013.60
  • EUR 811.20
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) CLIA Kit

20-abx493534
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

20-abx258522
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Ra-10x96wellstestplate 10x96-wells test plate
EUR 6149.04
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in Tissue homogenates, cell lysates and other biological fluids.

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Ra-1x48wellstestplate 1x48-wells test plate
EUR 611.57
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in Tissue homogenates, cell lysates and other biological fluids.

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Ra-1x96wellstestplate 1x96-wells test plate
EUR 822.24
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in Tissue homogenates, cell lysates and other biological fluids.

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Ra-5x96wellstestplate 5x96-wells test plate
EUR 3340.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in Tissue homogenates, cell lysates and other biological fluids.

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

4-SEC199Ra
  • EUR 6210.00
  • EUR 3280.80
  • EUR 823.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

20-abx258521
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Protein Phosphatase 1 Regulatory Subunit 15A (PPP1R15A) ELISA Kit

abx259580-96tests 96 tests
EUR 1093.2

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) CLIA Kit

20-abx493533
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Mu-10x96wellstestplate 10x96-wells test plate
EUR 5834.88
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in tissue homogenates, cell lysates and other biological fluids.

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Mu-1x48wellstestplate 1x48-wells test plate
EUR 585.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in tissue homogenates, cell lysates and other biological fluids.

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Mu-1x96wellstestplate 1x96-wells test plate
EUR 785.28
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in tissue homogenates, cell lysates and other biological fluids.

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Mu-5x96wellstestplate 5x96-wells test plate
EUR 3173.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in tissue homogenates, cell lysates and other biological fluids.

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

4-SEC199Mu
  • EUR 5895.60
  • EUR 3114.00
  • EUR 786.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Protein Phosphatase 1, Regulatory Subunit 15A ELISA Kit (PPP1R15A)

RK03130 96 Tests
EUR 625.2

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K6981706 1.0 ug DNA
EUR 200.4

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K6981707 1.0 ug DNA
EUR 200.4

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K6981708 1.0 ug DNA
EUR 200.4

PPP1R15A sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K1706806 1.0 ug DNA
EUR 200.4

PPP1R15A sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K1706807 1.0 ug DNA
EUR 200.4

PPP1R15A sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K1706808 1.0 ug DNA
EUR 200.4

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3806506 1.0 ug DNA
EUR 200.4

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3806507 1.0 ug DNA
EUR 200.4

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3806508 1.0 ug DNA
EUR 200.4

PPP1R15A Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV654782 1.0 ug DNA
EUR 818.4

PPP1R15A Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV654783 1.0 ug DNA
EUR 888

PPP1R15A Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV654784 1.0 ug DNA
EUR 888

ELISA kit for Rat PPP1R15A (Protein Phosphatase 1, Regulatory Subunit 15A)

ELK7614 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Protein Phosphatase 1, Regulatory Subunit 15A from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat)

4-PAC199Ra01
  • EUR 310.80
  • EUR 3249.60
  • EUR 804.00
  • EUR 393.60
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A)

ELISA kit for Mouse PPP1R15A (Protein Phosphatase 1, Regulatory Subunit 15A)

ELK7615 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Protein Phosphatase 1, Regulatory Subunit 15A from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse)

4-PAC199Mu01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A)

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), PE

4-PAC199Ra01-PE
  • EUR 373.20
  • EUR 3427.20
  • EUR 964.80
  • EUR 471.60
  • EUR 242.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with PE.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), PE

4-PAC199Mu01-PE
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with PE.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), APC

4-PAC199Ra01-APC
  • EUR 436.80
  • EUR 4254.00
  • EUR 1176.00
  • EUR 560.40
  • EUR 272.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with APC.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), Cy3

4-PAC199Ra01-Cy3
  • EUR 532.80
  • EUR 5622.00
  • EUR 1518.00
  • EUR 697.20
  • EUR 313.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with Cy3.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), FITC

4-PAC199Ra01-FITC
  • EUR 373.20
  • EUR 3427.20
  • EUR 964.80
  • EUR 471.60
  • EUR 242.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with FITC.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), HRP

4-PAC199Ra01-HRP
  • EUR 398.40
  • EUR 3706.80
  • EUR 1039.20
  • EUR 505.20
  • EUR 255.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with HRP.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), APC

4-PAC199Mu01-APC
  • EUR 421.20
  • EUR 4038.00
  • EUR 1122.00
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with APC.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), Cy3

4-PAC199Mu01-Cy3
  • EUR 512.40
  • EUR 5334.00
  • EUR 1446.00
  • EUR 668.40
  • EUR 304.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with Cy3.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), FITC

4-PAC199Mu01-FITC
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with FITC.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), HRP

4-PAC199Mu01-HRP
  • EUR 385.20
  • EUR 3519.60
  • EUR 992.40
  • EUR 486.00
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with HRP.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), Biotinylated

4-PAC199Ra01-Biotin
  • EUR 390.00
  • EUR 3189.60
  • EUR 932.40
  • EUR 480.00
  • EUR 270.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with Biotin.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), APC-Cy7

4-PAC199Ra01-APC-Cy7
  • EUR 729.60
  • EUR 8364.00
  • EUR 2208.00
  • EUR 976.80
  • EUR 402.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with APC-Cy7.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), Biotinylated

4-PAC199Mu01-Biotin
  • EUR 379.20
  • EUR 3031.20
  • EUR 892.80
  • EUR 464.40
  • EUR 265.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with Biotin.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), APC-Cy7

4-PAC199Mu01-APC-Cy7
  • EUR 699.60
  • EUR 7932.00
  • EUR 2100.00
  • EUR 933.60
  • EUR 388.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with APC-Cy7.

Ppp1r14a/ Rat Ppp1r14a ELISA Kit

ELI-36538r 96 Tests
EUR 1063.2

Ppp1r12a/ Rat Ppp1r12a ELISA Kit

ELI-44016r 96 Tests
EUR 1063.2

PPP2R1A/PPP2R2A/PPP2R1B Rabbit pAb

A18571-100ul 100 ul
EUR 369.6

PPP2R1A/PPP2R2A/PPP2R1B Rabbit pAb

A18571-200ul 200 ul
EUR 550.8

PPP2R1A/PPP2R2A/PPP2R1B Rabbit pAb

A18571-20ul 20 ul
EUR 219.6

PPP2R1A/PPP2R2A/PPP2R1B Rabbit pAb

A18571-50ul 50 ul
EUR 267.6

Ppp1r1a/ Rat Ppp1r1a ELISA Kit

ELI-05382r 96 Tests
EUR 788.4

Ppp2r2a/ Rat Ppp2r2a ELISA Kit

ELI-12267r 96 Tests
EUR 1063.2

Ppp1r9a/ Rat Ppp1r9a ELISA Kit

ELI-20686r 96 Tests
EUR 1063.2

PPP1R12A siRNA

20-abx929463
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

PPP1R12A siRNA

20-abx929464
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

PPP1R14A siRNA

20-abx929473
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

PPP1R14A siRNA

20-abx929474
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

PPP1R16A siRNA

20-abx929485
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

PPP1R16A siRNA

20-abx929486
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

PPP1R12A siRNA

20-abx904176
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

PPP1R14A siRNA

20-abx904177
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

PPP1R14A Rabbit pAb

A10160-100ul 100 ul
EUR 369.6

PPP1R14A Rabbit pAb

A10160-200ul 200 ul
EUR 550.8

PPP1R14A Rabbit pAb

A10160-20ul 20 ul
EUR 219.6

PPP1R14A Rabbit pAb

A10160-50ul 50 ul
EUR 267.6

PPP1R12A Rabbit pAb

A0587-100ul 100 ul
EUR 369.6

PPP1R12A Rabbit pAb

A0587-200ul 200 ul
EUR 550.8

PPP1R12A Rabbit pAb

A0587-20ul 20 ul
EUR 219.6

PPP1R12A Rabbit pAb

A0587-50ul 50 ul
EUR 267.6

PPP1R12A Rabbit pAb

A6700-100ul 100 ul
EUR 369.6

PPP1R12A Rabbit pAb

A6700-200ul 200 ul
EUR 550.8

PPP1R12A Rabbit pAb

A6700-20ul 20 ul
EUR 219.6

PPP1R12A Rabbit pAb

A6700-50ul 50 ul
EUR 267.6

PPP1R14A antibody

10R-1093 100 ul
EUR 379.2
Description: Mouse monoclonal PPP1R14A antibody

PPP1R16A Antibody

39949-100ul 100ul
EUR 468

PPP1R14A Antibody

39990-100ul 100ul
EUR 468

PPP1R12A antibody

39113-100ul 100ul
EUR 302.4

PPP1R12A antibody

70R-10251 50 ug
EUR 560.4
Description: Affinity purified rabbit polyclonal PPP1R12A antibody

PPP1R16A antibody

70R-12898 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal PPP1R16A antibody

PPP1R12A antibody

70R-14218 100 ug
EUR 386.4
Description: Affinity purified Rabbit polyclonal PPP1R12A antibody

PPP1R14A antibody

70R-19471 50 ul
EUR 522
Description: Rabbit polyclonal PPP1R14A antibody

In addition, Ppme1 promoter active in muscle cells, while mutation of conserved E-box element prevents full induction Ppme1 reporter gene, suggesting that Ppme1 is transcriptionally regulated by myogenic regulatory factors. Interestingly, immunofluorescence analysis showed that Ppme1 localized to both the cytoplasm and nucleus, whereas cell fractionation showed that Ppme1 only found in the cytoplasm. Functional studies revealed that the inhibition of muscle cell differentiation Ppme1 use AMZ30 ABL127 or attenuates.

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