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SIRT2 is involved in cisplatin-induced acute kidney injury through regulation of mitogen-activated protein kinase phosphatase1.

SIRT2 is involved in cisplatin-induced acute kidney injury through regulation of mitogen-activated protein kinase phosphatase-1.
Posted by Karen

Activation of mitogen-activated protein kinase phosphatase-1 (MKP-1), dual-specificity protein phosphatase, set the mitogen-activated protein kinase signaling. C-Jun N-terminal kinase (JNK) and p38 activated cisplatin-induced renal injury. However, changes in the expression of MKP-1 cisplatin-induced kidney injury and the effects of regulation sirtuin 2 (SIRT2), a nicotinamide adenine dinucleotide dependent deacetylase, the MKP-1 remains unknown.

To solve this problem, we use the constitution Sirt2 knockout (KO) mice, transgenic (TG) mice with increased expression of SIRT2 particularly in the proximal tubule epithelial cellsand wild-type (WT) mice. Cisplatin nephrotoxicity induced by intraperitoneal injection of cisplatin.MKP-1 expression in the kidney to decline after cisplatin treatment. Cisplatin-induced downregulation of MKP-1 KO mice Sirt2 reversed in the kidneys and then decreased in TG mice Sirt2 kidney.

We observed a similar phenomenon with SIRT2-knockdown or SIRT2-expressed in tubular epithelial cells. Phosphorylation of p38 and JNK, downstream signaling pathways MKP-1, is increased in WT mice after treatment with cisplatin kidney. Decrease SIRT2 suppressed cisplatin-induced phosphorylation of p38 and JNK in renal and tubular epithelial cells.

Excess of SIRT2 increase phosphorylation of p38 and JNK in renal and tubular epithelial cells. Acetylation of MKP-1 increased significantly in SIRT2-knockdown cells and a decrease in SIRT2-expressed cells after stimulation cisplatin. Sirt2 KO mice and Sirt2 TG mice showed improvement and aggravation of kidney injury, apoptosis, necroptosis and inflammation caused by cisplatin.Our data showed that SIRT2 associated with cisplatin-induced renal injury through regulation MKP-1 expression.

SIRT2 is involved in cisplatin-induced acute kidney injury through regulation of mitogen-activated <em>protein</em> kinase <em>phosphatase</em>-<em>1</em>.
SIRT2 is involved in cisplatin-induced acute kidney injury through regulation of mitogen-activated protein kinase phosphatase1.

Protein phosphatase one controls the activity of a balance between collective and singular mode of cell migration.

collective cell migration is central to many developmental and pathological processes. However, the collective mechanisms that keep cells together and coordinate the movement of some cells are poorly understood. Using a Drosophila Model border cell migration, we found that cell cohesion protein phosphatase 1 (PP1) activity collective control and migration.

Inhibition of PP1 causes cells to get round the border, which split off, and move it as single cell motility changes. We are proof that PP1 promoting appropriate levels of cadherin-catenin protein-complex at the intersection of the cells in the cluster to keep the cells along the border. Limiting PP1 actomyosin contractility advanced to the outskirts of the cluster rather than on the internal border cell contact that individual. We showed that the myosin phosphatase PP1 complex, which blocks non-muscle myosin II- (Myo-II) activity, cell shape and the boundary coordinates of cluster cohesion.

Given the high conservation of the PP1 complex, this study identifies collective PP1 as the main regulator of the single cell migration.
Protein phosphatase methylesterase 1 has been identified as a new gene that is upregulated in skeletal muscle in response to neurogenic atrophy in mice. Western blot analysis confirms that Ppme1 expressed during both muscle cell proliferation and differentiation.

PPP1R15A Antibody

1-CSB-PA787946
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PPP1R15A. Recognizes PPP1R15A from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-2000, ELISA:1:10000-20000

PPP1R15A siRNA

20-abx929481
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

PPP1R15A siRNA

20-abx929482
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

PPP1R15A siRNA

20-abx904181
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

PPP1R15A Antibody

39948-100ul 100ul
EUR 390

PPP1R15A Antibody

43273-100ul 100ul
EUR 252

PPP1R15A antibody

10R-7104 100 ul
EUR 691
Description: Mouse monoclonal PPP1R15A antibody

PPP1R15A antibody

10R-7105 100 ul
EUR 691
Description: Mouse monoclonal PPP1R15A antibody

PPP1R15A antibody

10R-7106 100 ul
EUR 691
Description: Mouse monoclonal PPP1R15A antibody

PPP1R15A cloning plasmid

CSB-CL018528HU-10ug 10ug
EUR 677
Description: A cloning plasmid for the PPP1R15A gene.

Anti-PPP1R15A antibody

STJ118711 100 µl
EUR 277
Description: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation.

PPP1R15A Rabbit pAb

A16260-100ul 100 ul
EUR 308

PPP1R15A Rabbit pAb

A16260-200ul 200 ul
EUR 459

PPP1R15A Rabbit pAb

A16260-20ul 20 ul
EUR 183

PPP1R15A Rabbit pAb

A16260-50ul 50 ul
EUR 223

PPP1R15A Conjugated Antibody

C43273 100ul
EUR 397

Mouse PPP1R15A shRNA Plasmid

20-abx971639
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Rat PPP1R15A shRNA Plasmid

20-abx987671
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human PPP1R15A shRNA Plasmid

20-abx958419
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Anti-PPP1R15A / GADD34 antibody

STJ70012 100 µg
EUR 359

Human PPP1R15A ELISA KIT

ELI-43112h 96 Tests
EUR 824

Mouse Ppp1r15a ELISA KIT

ELI-43113m 96 Tests
EUR 865

Bovine PPP1R15A ELISA KIT

ELI-19724b 96 Tests
EUR 928

PPP1R15A Recombinant Protein (Human)

RP024352 100 ug Ask for price

PPP1R15A Recombinant Protein (Mouse)

RP163886 100 ug Ask for price

PPP1R15A Recombinant Protein (Rat)

RP221720 100 ug Ask for price

h PPP1R15A inducible lentiviral particles

LVP854 1x107 IFU/ml x 200ul
EUR 451
Description: Pre-made over-expression lentivirus for expressing human target: h PPP1R15A (protein phosphatase 1, regulatory subunit 15A), [alternative names: GADD34]. The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_014330.3 . It also contains a RFP-Blasticidin dual selection marker.

PPP1R15A ELISA Kit (Mouse) (OKCD00311)

OKCD00311 96 Wells
EUR 857
Description: Description of target: Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. In case of infection with vesicular stomatitis virus (VSV), impairs viral replication.5 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.6"Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha."_x005F_x005F_x000D_Novoa I., Zeng H., Harding H.P., Ron D._x005F_x005F_x000D_J. Cell Biol. 153:1011-1022(2001) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH PP1, MUTAGENESIS OF VAL-549.Ref.8"Stress-induced gene expression requires programmed recovery from translational repression."_x005F_x005F_x000D_Novoa I., Zhang Y., Zeng H., Jungreis R., Harding H.P., Ron D._x005F_x005F_x000D_EMBO J. 22:1180-1187(2003) [PubMed] [Europe PMC] [Abstract]Cited for: INDUCTION, FUNCTION.Ref.9"The function of GADD34 is a recovery from a shutoff of protein synthesis induced by ER stress: elucidation by GADD34-deficient mice."_x005F_x005F_x000D_Kojima E., Takeuchi A., Haneda M., Yagi A., Hasegawa T., Yamaki K., Takeda K., Akira S., Shimokata K., Isobe K._x005F_x005F_x000D_FASEB J. 17:1573-1575(2003) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.Ref.10"Gadd34 requirement for normal hemoglobin synthesis."_x005F_x005F_x000D_Patterson A.D., Hollander M.C., Miller G.F., Fornace A.J. Jr._x005F_x005F_x000D_Mol. Cell. Biol. 26:1644-1653(2006) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, DISRUPTION PHENOTYPE.Ref.11"Suppression of viral replication by stress-inducible GADD34 protein via the mammalian serine/threonine protein kinase mTOR pathway."_x005F_x005F_x000D_Minami K., Tambe Y., Watanabe R., Isono T., Haneda M., Isobe K., Kobayashi T., Hino O., Okabe H., Chano T., Inoue H._x005F_x005F_x000D_J. Virol. 81:11106-11115(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INDUCTION. ;Species reactivity: Mouse;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.057 ng/mL

PPP1R15A ELISA Kit (Rat) (OKCD00864)

OKCD00864 96 Wells
EUR 896
Description: Description of target: Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.1 Publication <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.6"Potential molecular mechanism for rodent tumorigenesis: mutational generation of Progression Elevated Gene-3 (PEG-3)."_x005F_x005F_x000D_Su Z.-Z., Emdad L., Sarkar D., Randolph A., Valerie K., Yacoub A., Dent P., Fisher P.B._x005F_x005F_x000D_Oncogene 24:2247-2255(2005) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, MUTANT PEG-3. ;Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.118 ng/mL

Ppp1r15a ORF Vector (Rat) (pORF)

ORF073908 1.0 ug DNA
EUR 506

PPP1R15A ORF Vector (Human) (pORF)

ORF008118 1.0 ug DNA
EUR 95

pECMV-Ppp1r15a-m-FLAG Plasmid

PVT14941 2 ug
EUR 325

Ppp1r15a ORF Vector (Mouse) (pORF)

ORF054630 1.0 ug DNA
EUR 506

Polyclonal Goat Anti-PPP1R15A / GADD34 Antibody

APG00250G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-PPP1R15A / GADD34 . This antibody is tested and proven to work in the following applications:

PPP1R15A sgRNA CRISPR Lentivector set (Human)

K1706801 3 x 1.0 ug
EUR 339

Ppp1r15a sgRNA CRISPR Lentivector set (Rat)

K6981701 3 x 1.0 ug
EUR 339

Ppp1r15a sgRNA CRISPR Lentivector set (Mouse)

K3806501 3 x 1.0 ug
EUR 339

PPP1R15A sgRNA CRISPR Lentivector (Human) (Target 1)

K1706802 1.0 ug DNA
EUR 154

PPP1R15A sgRNA CRISPR Lentivector (Human) (Target 2)

K1706803 1.0 ug DNA
EUR 154

PPP1R15A sgRNA CRISPR Lentivector (Human) (Target 3)

K1706804 1.0 ug DNA
EUR 154

Ppp1r15a sgRNA CRISPR Lentivector (Rat) (Target 1)

K6981702 1.0 ug DNA
EUR 154

Ppp1r15a sgRNA CRISPR Lentivector (Rat) (Target 2)

K6981703 1.0 ug DNA
EUR 154

Ppp1r15a sgRNA CRISPR Lentivector (Rat) (Target 3)

K6981704 1.0 ug DNA
EUR 154

PPP1R15A 3'UTR GFP Stable Cell Line

TU068671 1.0 ml
EUR 1394

Ppp1r15a sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3806502 1.0 ug DNA
EUR 154

Ppp1r15a sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3806503 1.0 ug DNA
EUR 154

Ppp1r15a sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3806504 1.0 ug DNA
EUR 154

PPP1R15A Protein Vector (Rat) (pPB-C-His)

PV295630 500 ng
EUR 603

PPP1R15A Protein Vector (Rat) (pPB-N-His)

PV295631 500 ng
EUR 603

PPP1R15A Protein Vector (Rat) (pPM-C-HA)

PV295632 500 ng
EUR 603

PPP1R15A Protein Vector (Rat) (pPM-C-His)

PV295633 500 ng
EUR 603

PPP1R15A Protein Vector (Human) (pPB-C-His)

PV032469 500 ng
EUR 329

PPP1R15A Protein Vector (Human) (pPB-N-His)

PV032470 500 ng
EUR 329

PPP1R15A Protein Vector (Human) (pPM-C-HA)

PV032471 500 ng
EUR 329

PPP1R15A Protein Vector (Human) (pPM-C-His)

PV032472 500 ng
EUR 329

PPP1R15A Protein Vector (Mouse) (pPB-C-His)

PV218518 500 ng
EUR 603

PPP1R15A Protein Vector (Mouse) (pPB-N-His)

PV218519 500 ng
EUR 603

PPP1R15A Protein Vector (Mouse) (pPM-C-HA)

PV218520 500 ng
EUR 603

PPP1R15A Protein Vector (Mouse) (pPM-C-His)

PV218521 500 ng
EUR 603

Ppp1r15a 3'UTR Luciferase Stable Cell Line

TU216657 1.0 ml Ask for price

PPP1R15A 3'UTR Luciferase Stable Cell Line

TU018671 1.0 ml
EUR 1394

Ppp1r15a 3'UTR GFP Stable Cell Line

TU266657 1.0 ml Ask for price

Ppp1r15a 3'UTR Luciferase Stable Cell Line

TU116813 1.0 ml Ask for price

Ppp1r15a 3'UTR GFP Stable Cell Line

TU166813 1.0 ml Ask for price

Human Protein phosphatase 1 regulatory subunit 15A (PPP1R15A)

1-CSB-YP018528HU
  • EUR 586.00
  • EUR 299.00
  • EUR 2172.00
  • EUR 900.00
  • EUR 1442.00
  • EUR 382.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Protein phosphatase 1 regulatory subunit 15A(PPP1R15A),partial expressed in Yeast

Recombinant Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A)

4-RPC199Mu01
  • EUR 404.64
  • EUR 211.00
  • EUR 1242.40
  • EUR 480.80
  • EUR 861.60
  • EUR 334.00
  • EUR 2956.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Protein Phosphatase 1, Regulatory Subunit 15A expressed in: E.coli

Recombinant Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A)

4-RPC199Ra01
  • EUR 404.64
  • EUR 211.00
  • EUR 1242.40
  • EUR 480.80
  • EUR 861.60
  • EUR 334.00
  • EUR 2956.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Protein Phosphatase 1, Regulatory Subunit 15A expressed in: E.coli

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Antibody

20-abx131495
  • EUR 453.00
  • EUR 133.00
  • EUR 1302.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Antibody

20-abx131496
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Phosphatase 1, Regulatory Subunit 15A (Ppp1r15a) Antibody

20-abx114830
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Antibody

abx036158-100ug 100 ug
EUR 391

Protein Phosphatase 1 Regulatory Subunit 15A (PPP1R15A) Antibody

abx412519-01mg 0.1 mg
EUR 801

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Antibody

abx430859-200ul 200 ul
EUR 384

Protein Phosphatase 1 Regulatory Subunit 15A (PPP1R15A) Antibody

abx233293-100ug 100 ug
EUR 509

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Antibody

20-abx325005
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

PPP1R15A Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV654781 1.0 ug DNA
EUR 682

PPP1R15A Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV654785 1.0 ug DNA
EUR 682

PPP1R15A Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV654786 1.0 ug DNA
EUR 682

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Protein

20-abx168834
  • EUR 578.00
  • EUR 258.00
  • EUR 1678.00
  • EUR 676.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Protein

20-abx168835
  • EUR 578.00
  • EUR 258.00
  • EUR 1678.00
  • EUR 676.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse)

4-PAC199Mu01
  • EUR 251.00
  • EUR 2576.00
  • EUR 640.00
  • EUR 316.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A)

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat)

4-PAC199Ra01
  • EUR 259.00
  • EUR 2708.00
  • EUR 670.00
  • EUR 328.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A)

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Mu-10x96wellstestplate 10x96-wells test plate
EUR 4862.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in tissue homogenates, cell lysates and other biological fluids.

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Mu-1x48wellstestplate 1x48-wells test plate
EUR 488.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in tissue homogenates, cell lysates and other biological fluids.

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Mu-1x96wellstestplate 1x96-wells test plate
EUR 654.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in tissue homogenates, cell lysates and other biological fluids.

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Mu-5x96wellstestplate 5x96-wells test plate
EUR 2644.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in tissue homogenates, cell lysates and other biological fluids.

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

4-SEC199Mu
  • EUR 4913.00
  • EUR 2595.00
  • EUR 655.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Ra-10x96wellstestplate 10x96-wells test plate
EUR 5124.2
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in Tissue homogenates, cell lysates and other biological fluids.

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Ra-1x48wellstestplate 1x48-wells test plate
EUR 509.64
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in Tissue homogenates, cell lysates and other biological fluids.

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Ra-1x96wellstestplate 1x96-wells test plate
EUR 685.2
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in Tissue homogenates, cell lysates and other biological fluids.

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

SEC199Ra-5x96wellstestplate 5x96-wells test plate
EUR 2783.4
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in Tissue homogenates, cell lysates and other biological fluids.

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

4-SEC199Ra
  • EUR 5175.00
  • EUR 2734.00
  • EUR 686.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Protein Phosphatase 1, Regulatory Subunit 15A ELISA Kit (PPP1R15A)

RK03130 96 Tests
EUR 521

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) CLIA Kit

20-abx493533
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) CLIA Kit

20-abx493534
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Protein Phosphatase 1 Regulatory Subunit 15A (PPP1R15A) ELISA Kit

abx259580-96tests 96 tests
EUR 911

Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

20-abx258521
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) ELISA Kit

20-abx258522
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

PPP1R15A sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K1706805 3 x 1.0 ug
EUR 376

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6981705 3 x 1.0 ug
EUR 376

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3806505 3 x 1.0 ug
EUR 376

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), APC

4-PAC199Mu01-APC
  • EUR 351.00
  • EUR 3365.00
  • EUR 935.00
  • EUR 449.00
  • EUR 222.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with APC.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), Biotinylated

4-PAC199Mu01-Biotin
  • EUR 316.00
  • EUR 2526.00
  • EUR 744.00
  • EUR 387.00
  • EUR 221.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with Biotin.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), Cy3

4-PAC199Mu01-Cy3
  • EUR 427.00
  • EUR 4445.00
  • EUR 1205.00
  • EUR 557.00
  • EUR 254.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with Cy3.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), FITC

4-PAC199Mu01-FITC
  • EUR 301.00
  • EUR 2712.00
  • EUR 768.00
  • EUR 379.00
  • EUR 197.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with FITC.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), HRP

4-PAC199Mu01-HRP
  • EUR 321.00
  • EUR 2933.00
  • EUR 827.00
  • EUR 405.00
  • EUR 209.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with HRP.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), PE

4-PAC199Mu01-PE
  • EUR 301.00
  • EUR 2712.00
  • EUR 768.00
  • EUR 379.00
  • EUR 197.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with PE.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), APC

4-PAC199Ra01-APC
  • EUR 364.00
  • EUR 3545.00
  • EUR 980.00
  • EUR 467.00
  • EUR 227.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with APC.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), Biotinylated

4-PAC199Ra01-Biotin
  • EUR 325.00
  • EUR 2658.00
  • EUR 777.00
  • EUR 400.00
  • EUR 225.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with Biotin.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), Cy3

4-PAC199Ra01-Cy3
  • EUR 444.00
  • EUR 4685.00
  • EUR 1265.00
  • EUR 581.00
  • EUR 261.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with Cy3.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), FITC

4-PAC199Ra01-FITC
  • EUR 311.00
  • EUR 2856.00
  • EUR 804.00
  • EUR 393.00
  • EUR 202.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with FITC.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), HRP

4-PAC199Ra01-HRP
  • EUR 332.00
  • EUR 3089.00
  • EUR 866.00
  • EUR 421.00
  • EUR 213.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with HRP.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), PE

4-PAC199Ra01-PE
  • EUR 311.00
  • EUR 2856.00
  • EUR 804.00
  • EUR 393.00
  • EUR 202.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with PE.

ELISA kit for Rat PPP1R15A (Protein Phosphatase 1, Regulatory Subunit 15A)

ELK7614 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Protein Phosphatase 1, Regulatory Subunit 15A from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

ELISA kit for Mouse PPP1R15A (Protein Phosphatase 1, Regulatory Subunit 15A)

ELK7615 1 plate of 96 wells
EUR 432
Description: A sandwich ELISA kit for detection of Protein Phosphatase 1, Regulatory Subunit 15A from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

PPP1R15A sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K1706806 1.0 ug DNA
EUR 167

PPP1R15A sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K1706807 1.0 ug DNA
EUR 167

PPP1R15A sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K1706808 1.0 ug DNA
EUR 167

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K6981706 1.0 ug DNA
EUR 167

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K6981707 1.0 ug DNA
EUR 167

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K6981708 1.0 ug DNA
EUR 167

PPP1R15A Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV654782 1.0 ug DNA
EUR 682

PPP1R15A Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV654783 1.0 ug DNA
EUR 740

PPP1R15A Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV654784 1.0 ug DNA
EUR 740

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3806506 1.0 ug DNA
EUR 167

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3806507 1.0 ug DNA
EUR 167

Ppp1r15a sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3806508 1.0 ug DNA
EUR 167

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Mouse), APC-Cy7

4-PAC199Mu01-APC-Cy7
  • EUR 583.00
  • EUR 6610.00
  • EUR 1750.00
  • EUR 778.00
  • EUR 324.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with APC-Cy7.

Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A) Polyclonal Antibody (Rat), APC-Cy7

4-PAC199Ra01-APC-Cy7
  • EUR 608.00
  • EUR 6970.00
  • EUR 1840.00
  • EUR 814.00
  • EUR 335.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Rat Protein Phosphatase 1, Regulatory Subunit 15A (PPP1R15A). This antibody is labeled with APC-Cy7.

In addition, Ppme1 promoter active in muscle cells, while mutation of conserved E-box element prevents full induction Ppme1 reporter gene, suggesting that Ppme1 is transcriptionally regulated by myogenic regulatory factors. Interestingly, immunofluorescence analysis showed that Ppme1 localized to both the cytoplasm and nucleus, whereas cell fractionation showed that Ppme1 only found in the cytoplasm. Functional studies revealed that the inhibition of muscle cell differentiation Ppme1 use AMZ30 ABL127 or attenuates.

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